ClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorCardoso, Valdemir M.-
Autor(es): dc.creatorParedes, Sheyla A. H.-
Autor(es): dc.creatorCampani, Gilson-
Autor(es): dc.creatorGonçalves, Viviane M.-
Autor(es): dc.creatorZangirolami, Teresa C.-
Data de aceite: dc.date.accessioned2026-02-09T12:44:53Z-
Data de disponibilização: dc.date.available2026-02-09T12:44:53Z-
Data de envio: dc.date.issued2022-08-09-
Data de envio: dc.date.issued2022-08-09-
Data de envio: dc.date.issued2021-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/50907-
Fonte completa do material: dc.identifierhttps://link.springer.com/article/10.1007/s00253-022-11758-9-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1167483-
Descrição: dc.descriptionSeveral studies have searched for new antigens to produce pneumococcal vaccines that are more effective and could provide broader coverage, given the great number of serotypes causing pneumococcal diseases. One of the promising subunit vaccine candidates is untagged recombinant pneumococcal surface protein A (PspA4Pro), obtainable in high quantities using recombinant Escherichia coli as a microbial factory. However, lipopolysaccharides (LPS) present in E. coli cell extracts must be removed, in order to obtain the target protein at the required purity, which makes the downstream process more complex and expensive. Endotoxin-free E. coli strains, which synthesize a nontoxic mutant LPS, may offer a cost-effective alternative way to produce recombinant proteins for application as therapeutics. This paper presents an investigation of PspA4Pro production employing the endotoxin-free recombinant strain ClearColi® BL21(DE3) with different media (defined, auto-induction, and other complex media), temperatures (27, 32, and 37 °C), and inducers. In comparison to conventional E. coli cells in a defined medium, ClearColi presented similar PspA4Pro yields, with lower productivities. Complex medium formulations supplemented with salts favored PspA4Pro yields, titers, and ClearColi growth rates. Induction with isopropyl-β-D-thiogalactopyranoside (0.5 mM) and lactose (2.5 g/L) together in a defined medium at 32 °C, which appeared to be a promising cultivation strategy, was reproduced in 5 L bioreactor culture, leading to a yield of 146.0 mg PspA4Pro/g dry cell weight. After purification, the cell extract generated from ClearColi led to 98% purity PspA4Pro, which maintained secondary structure and biological function. ClearColi is a potential host for industrial recombinant protein production.-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceApplied Microbiology and Biotechnology-
Palavras-chave: dc.subjectBioprocess engineering-
Palavras-chave: dc.subjectClearColi-
Palavras-chave: dc.subjectEndotoxin-free Escherichia coli-
Palavras-chave: dc.subjectProcess conditions-
Palavras-chave: dc.subjectUpstream-
Palavras-chave: dc.subjectRecombinant protein-
Título: dc.titleClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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