Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorRamos, Cíntia Lacerda-
Autor(es): dc.creatorThorsen, Line-
Autor(es): dc.creatorRyssel, Mia-
Autor(es): dc.creatorNielsen, Dennis S.-
Autor(es): dc.creatorSiegumfeldt, Henrik-
Autor(es): dc.creatorSchwan, Rosane Freitas-
Autor(es): dc.creatorJespersen, Lene-
Data de aceite: dc.date.accessioned2026-02-09T12:42:05Z-
Data de disponibilização: dc.date.available2026-02-09T12:42:05Z-
Data de envio: dc.date.issued2020-05-10-
Data de envio: dc.date.issued2020-05-10-
Data de envio: dc.date.issued2014-04-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/40757-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0923250814000278-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1166557-
Descrição: dc.descriptionIn the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pHi closer to the extracellular pH (pHex) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pHex of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceResearch in Microbiology-
Palavras-chave: dc.subjectLactobacillus brevis-
Palavras-chave: dc.subjectLactobacillus plantarum-
Palavras-chave: dc.subjectIn vitro gastro-intestinal model-
Palavras-chave: dc.subjectpHi-
Palavras-chave: dc.subjectFRIM-
Palavras-chave: dc.subjectProbiotic-
Palavras-chave: dc.subjectIntracellular pH-
Palavras-chave: dc.subjectFluorescence ratio imaging microscopy (FRIM)-
Título: dc.titleEffect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

Não existem arquivos associados a este item.