Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorIshige, Takayuki-
Autor(es): dc.creatorMurata, Shota-
Autor(es): dc.creatorTaniguchi, Toshibumi-
Autor(es): dc.creatorMiyabe, Akiko-
Autor(es): dc.creatorKitamura, Kouichi-
Autor(es): dc.creatorKawasaki, Kenji-
Autor(es): dc.creatorNishimura, Motoi-
Autor(es): dc.creatorIgari, Hidetoshi-
Autor(es): dc.creatorMatsushita, Kazuyuki-
Data de aceite: dc.date.accessioned2026-02-09T12:38:34Z-
Data de disponibilização: dc.date.available2026-02-09T12:38:34Z-
Data de envio: dc.date.issued2020-08-20-
Data de envio: dc.date.issued2020-08-20-
Data de envio: dc.date.issued2020-08-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/42499-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0009898120301789?via%3Dihub-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1165390-
Descrição: dc.descriptionBackground The detection of SARS-CoV-2 RNA by real-time reverse transcription–polymerase chain reaction (rRT-PCR) is used to confirm the clinical diagnosis of COVID-19 by molecular diagnostic laboratories. We developed a multiplex rRT-PCR methodology for the detection of SARS-CoV-2 RNA. Methods Three genes were used for multiplex rRT-PCR: the Sarbecovirus specific E gene, the SARS-CoV-2 specific N gene, and the human ABL1 gene as an internal control. Results Good correlation of Cq values was observed between the simplex and multiplex rRT-PCR methodologies. Low copies (<25 copies/reaction) of SARS-CoV-2 RNA were detected by the novel multiplex rRT-PCR method. Conclusion The proposed multiplex rRT-PCR methodology will enable highly sensitive detection of SARS-CoV-2 RNA, reducing reagent use and cost, and time required by clinical laboratory technicians.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceClinica Chimica Acta-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-
Palavras-chave: dc.subjectMolecular diagnosis-
Palavras-chave: dc.subjectMultiplex-
Palavras-chave: dc.subjectReal-time reverse transcription-polymerase chain reaction (rRT-PCR)-
Palavras-chave: dc.subjectHigh-sensitive-
Título: dc.titleHighly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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