Effects of extenders, cryoprotectants and freezing methods on sperm quality of the threatened Brazilian freshwater fish pirapitinga-do-sul Brycon opalinus (Characiformes)

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorViveiros, Ana Tereza de Mendonça-
Autor(es): dc.creatorOrfão, Laura Helena-
Autor(es): dc.creatorNascimento, Ariane Flávia do-
Autor(es): dc.creatorCorrêa, Fábio Mathias-
Autor(es): dc.creatorCaneppele, Danilo-
Data de aceite: dc.date.accessioned2026-02-09T12:37:32Z-
Data de disponibilização: dc.date.available2026-02-09T12:37:32Z-
Data de envio: dc.date.issued2016-12-19-
Data de envio: dc.date.issued2016-12-19-
Data de envio: dc.date.issued2016-12-19-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/12091-
Fonte completa do material: dc.identifierhttp://www.theriojournal.com/article/S0093-691X(12)00106-9/pdf-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1165060-
Descrição: dc.descriptionThe objective was to develop a suitable freezing method to cryopreserve Brycon opalinus (Characiformes) sperm. Extenders (NaCl and glucose at 325 and 365 mOsm/kg), cryoprotectants (dimethyl sulfoxide=dimethyl sulfoxide (DMSO) and methyl glycol=methyl glycol (MG)), equilibration times (15 and 30 min), thawing temperatures (30 and 60 °C), and straw sizes (0.5 and 4.0 mL) were tested. Sperm were frozen in a liquid nitrogen vapor vessel at -170 °C and subsequently stored in liquid nitrogen. Post-thaw sperm quality was always evaluated in terms of motility (expressed as percentage of motile sperm), duration of motility and vitality (eosin-nigrosin staining, expressed as percentage of intact sperm). The best freezing method was also tested for fertility and hatching (expressed as the percentage of fertilized eggs). Post-thaw sperm quality was highest when sperm were cryopreserved in Glucose 365 mOsm/kg and MG, after a 30-min equilibration and thawed at 60 °C for 8 s, of regardless straw size: 74±7% motile sperm, 47±4 s of motility duration, 69±3% intact sperm, 64±4% fertilization and 63±3% hatching. The freezing method developed in the present study was efficient and can be used to maximize larvae production for both aquaculture purposes and for conservational programs, since B. opalinus is a threatened species.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceTheriogenology-
Título: dc.titleEffects of extenders, cryoprotectants and freezing methods on sperm quality of the threatened Brazilian freshwater fish pirapitinga-do-sul Brycon opalinus (Characiformes)-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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