Viability of Campylobacter spp. in frozen and chilled broiler carcasses according to real-time PCR with propidium monoazide pretreatment

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorCastro, Andréa G. S. A.-
Autor(es): dc.creatorDorneles, Elaine M. S.-
Autor(es): dc.creatorSantos, Ethiene L. S.-
Autor(es): dc.creatorAlves, Telma M.-
Autor(es): dc.creatorSilva, Guilherme R.-
Autor(es): dc.creatorFigueiredo, Tadeu C.-
Autor(es): dc.creatorAssis, Débora C. S.-
Autor(es): dc.creatorLage, Andrey P.-
Autor(es): dc.creatorCançado, Silvana V.-
Data de aceite: dc.date.accessioned2026-02-09T12:37:04Z-
Data de disponibilização: dc.date.available2026-02-09T12:37:04Z-
Data de envio: dc.date.issued2019-04-01-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2019-04-01-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2018-05-01-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57717-
Fonte completa do material: dc.identifierhttps://academic.oup.com/ps/article-abstract/97/5/1706/4876955?redirectedFrom=fulltext-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1164903-
Descrição: dc.descriptionThe aim of this study was to evaluate the viability of Campylobacter spp. in frozen and chilled broiler carcasses using real-time PCR with propidium monoazide (PMA) pretreatment. Sixty broiler carcasses were collected: 30 frozen and 30 chilled. Each carcass was submitted to 2 real-time PCR protocols to detect and quantify Campylobacter spp.: one using pretreatment with PMA, which blocks the amplification of DNA from dead bacteria, and the other without PMA. The results showed that PMA-pretreated carcasses, either frozen or chilled, had a lower positivity rate compared to untreated samples (P < 0.001). Regarding storage temperatures, PMA-pretreated frozen carcasses that tested positive were in a lesser number than chilled carcasses (P < 0.05). However, the quantification of total and live bacteria in PMA-pretreated frozen carcasses that tested positive showed no significant difference compared to chilled carcasses. It was concluded that the real-time PCR with PMA pretreatment was a sensitive method for evaluating the viability of Campylobacter spp. in broiler carcasses. Chilled broiler carcasses would represent greater hazard to public health concerning Campylobacter transmission.-
Idioma: dc.languageen-
Publicador: dc.publisherOxford Academic-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourcePoultry Science-
Palavras-chave: dc.subjectFood safety-
Palavras-chave: dc.subjectCampylobacter transmission-
Palavras-chave: dc.subjectPropidium monoazide-
Palavras-chave: dc.subjectSegurança alimentar-
Palavras-chave: dc.subjectTransmissão de campylobacter-
Palavras-chave: dc.subjectMonazida de propídio-
Título: dc.titleViability of Campylobacter spp. in frozen and chilled broiler carcasses according to real-time PCR with propidium monoazide pretreatment-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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