Influence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorOliveira, Kamila Ellen Souza de-
Autor(es): dc.creatorSouza, Rafaeli Aparecida Vieira de-
Autor(es): dc.creatorCarvalho, Lara Siqueira Oliveira-
Autor(es): dc.creatorPaiva, Luciano Vilela-
Data de aceite: dc.date.accessioned2026-02-09T12:36:56Z-
Data de disponibilização: dc.date.available2026-02-09T12:36:56Z-
Data de envio: dc.date.issued2022-10-30-
Data de envio: dc.date.issued2022-10-30-
Data de envio: dc.date.issued2022-06-10-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/55370-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1164861-
Descrição: dc.descriptionThe conservation of plant genetic resources is fundamental for the development of agriculture. The development of efficient cryopreservation protocols has become an effective tool to preserve cells, tissues and organs of different plant species. We sought here to develop an efficient method of cryopreservation of Eucalyptus grandis employing the V cryo-plate technique. This experiment examined the exposure of shoot tips to three different cryoprotectants (PVS2, PVS3, and PVS4). The cryoprotectants PVS2 and PVS4 proved to be more efficient among the cryoprotectants tested, as 44.4% of the shoot tips immersed in those solutions survived, and 31.1% generated new shoots. Ethylene glycol was found to be a key compound for the successful cryopreservation of shoot tips, in light of its low toxicity as compared to other cryoprotective compounds. Thus, the methodology developed here represents an effective method for the conservation of E. grandis germplasm through cryopreservation and the use of the V cryo-plate technique.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherUniversidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT)-
Direitos: dc.rightsAttribution 4.0 International-
Direitos: dc.rightsAttribution 4.0 International-
Direitos: dc.rightsacesso aberto-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
???dc.source???: dc.sourceCrop Breeding and Applied Biotechnology (CBAB)-
Palavras-chave: dc.subjectPlant vitrification solution-
Palavras-chave: dc.subjectGenetic conservation-
Palavras-chave: dc.subjectGermplasm-
Título: dc.titleInfluence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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