A universal design of betacoronavirus vaccines against COVID-19, MERS, and SARS

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Autor(es): dc.creatorDai, Lianpan-
Autor(es): dc.creatorZheng, Tianyi-
Autor(es): dc.creatorXu, Kun-
Autor(es): dc.creatorHan, Yuxuan-
Autor(es): dc.creatorXu, Lili-
Autor(es): dc.creatorHuang, Enqi-
Autor(es): dc.creatorAn, Yaling-
Autor(es): dc.creatorCheng, Yingjie-
Autor(es): dc.creatorLi, Shihua-
Autor(es): dc.creatorLiu, Mei-
Autor(es): dc.creatorYang, Mi-
Autor(es): dc.creatorLi, Yan-
Autor(es): dc.creatorCheng, Huijun-
Autor(es): dc.creatorYuan, Yuan-
Autor(es): dc.creatorZhang, Wei-
Autor(es): dc.creatorKe, Changwen-
Autor(es): dc.creatorWong, Gary-
Autor(es): dc.creatorQi, Jianxun-
Autor(es): dc.creatorQin, Chuan-
Autor(es): dc.creatorYan, Jinghua-
Autor(es): dc.creatorGao, George F.-
Data de aceite: dc.date.accessioned2026-02-09T12:36:26Z-
Data de disponibilização: dc.date.available2026-02-09T12:36:26Z-
Data de envio: dc.date.issued2020-08-03-
Data de envio: dc.date.issued2020-08-03-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/42181-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0092867420308126-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1164685-
Descrição: dc.descriptionVaccines are urgently needed to control the ongoing pandemic COVID-19 and previously emerging MERS/SARS caused by coronavirus (CoV) infections. The CoV spike receptor-binding domain (RBD) is an attractive vaccine target but is undermined by limited immunogenicity. We describe a dimeric form of MERS-CoV RBD that overcomes this limitation. The RBD-dimer significantly increased neutralizing antibody (NAb) titers compared to conventional monomeric form and protected mice against MERS-CoV infection. Crystal structure showed RBD-dimer fully exposed dual receptor-binding motifs, the major target for NAbs. Structure-guided design further yielded a stable version of RBD-dimer as a tandem repeat single-chain (RBD-sc-dimer) which retained the vaccine potency. We generalized this strategy to design vaccines against COVID-19 and SARS, achieving 10- to 100-fold enhancement of NAb titers. RBD-sc-dimers in pilot scale production yielded high yields, supporting their scalability for further clinical development. The framework of immunogen design can be universally applied to other beta-CoV vaccines to counter emerging threats.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
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Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-
Palavras-chave: dc.subjectMiddle East respiratory syndrome CoV (MERS-CoV)-
Palavras-chave: dc.subjectCoronavirus-
Palavras-chave: dc.subjectBetacoronavirus-
Palavras-chave: dc.subjectVaccine-
Palavras-chave: dc.subjectReceptor-binding domain (RBD)-
Título: dc.titleA universal design of betacoronavirus vaccines against COVID-19, MERS, and SARS-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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