Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorGuimarães, S. S. C.-
Autor(es): dc.creatorMatteoli, F. P.-
Autor(es): dc.creatorSilva, F. P.-
Autor(es): dc.creatorMatos, K. S.-
Autor(es): dc.creatorPfenning, L. H.-
Data de aceite: dc.date.accessioned2026-02-09T12:32:02Z-
Data de disponibilização: dc.date.available2026-02-09T12:32:02Z-
Data de envio: dc.date.issued2022-01-25-
Data de envio: dc.date.issued2022-01-25-
Data de envio: dc.date.issued2021-05-24-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/49029-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1163215-
Descrição: dc.descriptionMating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses.-
Descrição: dc.descriptionGUIMARÃES, S. S. C. et al. Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs. Current Research in Environmental & Applied Mycology, [S.l.], v. 11, n. 1, p. 159-170, 2021.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherBeijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection-
Direitos: dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International-
Direitos: dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International-
Direitos: dc.rightsacesso aberto-
Direitos: dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/-
Direitos: dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/-
???dc.source???: dc.sourceCurrent Research in Environmental & Applied Mycology (CREAM)-
Palavras-chave: dc.subjectHeterothallic-
Palavras-chave: dc.subjectHomothallic-
Palavras-chave: dc.subjectMAT primers-
Palavras-chave: dc.subjectNeocosmospora-
Palavras-chave: dc.subjectSexual reproduction-
Palavras-chave: dc.subjectWhole-genome-
Título: dc.titleStructural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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