Rapid extraction of melamine in powdered milk for direct electrospray ionization tandem mass spectrometry analysis

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorDomingo, Elisângela do Carmo-
Autor(es): dc.creatorTireli, Aline Auxiliadora-
Autor(es): dc.creatorNunes, Cleiton Antonio-
Autor(es): dc.creatorBatista, Alexandre Vieira-
Autor(es): dc.creatorGuerreiro, Mário César-
Autor(es): dc.creatorPinto, Sandra Maria-
Data de aceite: dc.date.accessioned2026-02-09T12:31:51Z-
Data de disponibilização: dc.date.available2026-02-09T12:31:51Z-
Data de envio: dc.date.issued2017-07-31-
Data de envio: dc.date.issued2017-07-31-
Data de envio: dc.date.issued2015-01-15-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/13451-
Fonte completa do material: dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0039914014008297?via%3Dihub-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1163150-
Descrição: dc.descriptionA combination of a simple pretreatment for melamine extraction and direct analysis in electrospray ionization tandem mass spectrometry (ESI–MS/MS) is proposed. Three pretreatments were evaluated. The first was based on suppressing interference using acetonitrile. The second used sulphuric acid and trichloroacetic acid to suppress interference and for melamine extraction, respectively. The third used sulphuric acid to suppress milk interference, trichloroacetic acid for melamine precipitation, and ethyl acetate for melamine extraction. However, only the last pretreatment suppressed milk interference in melamine detection and a good linearity (R2=0.99) was obtained. The presence of MS/MS 85 on melamine fragmentation spectrum showed the selectivity of this method. The limit of detection and limit of quantification were 0.269 µg L−1 and 0.897 µg L−1, respectively. The recoveries and relative standard deviation (RDS) of method were lower than 114% and 7.86%, respectively. Further, the research was extended to elucidate the nature of the melamine in the extract through infrared spectroscopy and microscopy analyses. The precipitate was characterized as melaminium bis(trichloroacetate) dihydrate, which is generated through hydrogen bound formation in an interaction between melamine and trichloroacetic acid. Therefore, a simple, fast, and easy method for melamine extraction and direct ESI–MS/MS analysis was developed.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceTalanta-
Palavras-chave: dc.subjectMelamine extraction-
Palavras-chave: dc.subjectTrichloroacetic acid precipitation-
Palavras-chave: dc.subjectMilk - Adulteration-
Palavras-chave: dc.subjectEthyl acetate extraction-
Palavras-chave: dc.subjectExtração de melamina-
Palavras-chave: dc.subjectPrecipitação de ácido tricloroacético-
Palavras-chave: dc.subjectLeite - Adulteração-
Palavras-chave: dc.subjectExtração de acetato de etilo-
Título: dc.titleRapid extraction of melamine in powdered milk for direct electrospray ionization tandem mass spectrometry analysis-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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