Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorKoblitz, Maria Gabriela Bello-
Autor(es): dc.creatorPastore, Gláucia Maria-
Data de aceite: dc.date.accessioned2026-02-09T12:25:27Z-
Data de disponibilização: dc.date.available2026-02-09T12:25:27Z-
Data de envio: dc.date.issued2006-06-01-
Data de envio: dc.date.issued2015-04-30-
Data de envio: dc.date.issued2015-04-30-
Data de envio: dc.date.issued2015-04-30-
Fonte completa do material: dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/6226-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1161021-
Descrição: dc.descriptionThe present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.-
Formato: dc.formattext/html-
Publicador: dc.publisherEditora da Universidade Federal de Lavras-
???dc.source???: dc.sourceCiência e Agrotecnologia v.30 n.3 2006-
Palavras-chave: dc.subjectLipase-
Palavras-chave: dc.subjectRhizopus sp.-
Palavras-chave: dc.subjectPurification-
Palavras-chave: dc.subjectBiochemical characterization-
Título: dc.titlePurification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.-
Tipo de arquivo: dc.typejournal article-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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