Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorSantos, Marta Maria Oliveira dos-
Autor(es): dc.creatorGama, Rafaela Santos-
Autor(es): dc.creatorTavares, Iasnaia Maria de Carvalho-
Autor(es): dc.creatorSantos, Pedro Henrique-
Autor(es): dc.creatorGonçalves, Márcia Soares-
Autor(es): dc.creatorCarvalho, Marise Silva de-
Autor(es): dc.creatorVilas Boas, Eduardo Valério de Barros-
Autor(es): dc.creatorOliveira, Julieta Rangel de-
Autor(es): dc.creatorMendes, Adriano Aguiar-
Autor(es): dc.creatorFranco, Marcelo-
Data de aceite: dc.date.accessioned2026-02-09T12:20:21Z-
Data de disponibilização: dc.date.available2026-02-09T12:20:21Z-
Data de envio: dc.date.issued2021-07-08-
Data de envio: dc.date.issued2021-07-08-
Data de envio: dc.date.issued2020-05-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/46697-
Fonte completa do material: dc.identifierhttps://doi.org/10.1002/bab.1959-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1159293-
Descrição: dc.descriptionThe present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus, and Candida sp. on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later applied to the synthesis of aromatic esters of apple peel and citrus (hexyl butyrate), apple and rose (geranyl butyrate), and apricot and pineapple (propyl butyrate). Scanning electron microscopy and gel electrophoresis confirmed a selective adsorption of lipases on Diaion, thus endorsing simultaneous immobilization and purification. Gibbs free energy (∆G) evinced the spontaneity of the process (–17.9 kJ/mol ≤ ∆G ≤ –5.1 kJ/mol). Maximum immobilized protein concentration of 30 mg/g support by CRL. This biocatalyst was the most active in olive oil hydrolysis (hydrolytic activity of 126.0 ± 2.0 U/g) and in the synthesis of aromatic esters. Maximum conversion yield of 89.1% was attained after 150 Min for the synthesis of hexyl butyrate, followed by the synthesis of geranyl butyrate (87.3% after 240 Min) and propyl butyrate (80.0% after 150 Min). CRL immobilized on Diaion retained around 93% of its original activity after six consecutive cycles of 150 Min for the synthesis of hexyl butyrate.-
Idioma: dc.languageen-
Publicador: dc.publisherInternational Union of Biochemistry and Molecular Biology-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceBiotechnology and Applied Biochemistry-
Palavras-chave: dc.subjectBiocatalysis-
Palavras-chave: dc.subjectLipase immobilization-
Palavras-chave: dc.subjectAroma synthesis-
Palavras-chave: dc.subjectBiocatálise-
Palavras-chave: dc.subjectImobilização de lipase-
Palavras-chave: dc.subjectSíntese de ésteres de aromas-
Título: dc.titleApplication of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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