Probiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorFonseca, Hugo Calixto-
Autor(es): dc.creatorMelo, Dirceu de Sousa-
Autor(es): dc.creatorRamos, Cíntia Lacerda-
Autor(es): dc.creatorDias, Disney Ribeiro-
Autor(es): dc.creatorSchwan, Rosane Freitas-
Data de aceite: dc.date.accessioned2026-02-09T12:20:03Z-
Data de disponibilização: dc.date.available2026-02-09T12:20:03Z-
Data de envio: dc.date.issued2020-08-19-
Data de envio: dc.date.issued2020-08-19-
Data de envio: dc.date.issued2020-05-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/42482-
Fonte completa do material: dc.identifierhttps://doi.org/10.1007/s12602-020-09659-2-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1159199-
Descrição: dc.descriptionWe evaluated the probiotic properties of lactic acid bacteria using resistance, safety, and functional assays. A preliminary subtractive screening of nineteen strains was performed based on their survival in simulated gastric and intestinal juice, and cell surface characteristics (hydrophobicity and auto-aggregation). Five strains were selected for further characterization, which included the assessment of their co-aggregation to pathogens, phenol tolerance, antimicrobial activity, and safety. Moreover, their adhesion to Caco-2 and HT-29 cells and the ability to inhibit pathogenic bacteria adhesion were evaluated. All strains had high (≥ 80.0%) survival rates in gastric and intestinal juices. Among them, Lactobacillus brevis CCMA 1284, L. plantarum CCMA 0743, and L. plantarum CCMA 0359 exhibited higher hydrophobicity (95.33, 96.06, and 80.02%, respectively), while L. paracasei CCMA 0504 and L. paracasei CCMA 0505 had the highest auto-aggregation values (45.36 and 52.66%, respectively). However, these last two strains were positive for the DNAse test, which is a safety concern. The CCMA 0359 and CCMA 1284 strains did not show antimicrobial activity, while the CCMA 0505 strain had a higher percentage of adhesion (4.75%) to Caco-2 cells. In the simulated competition and exclusion assays, the CCMA 0743 strain was able to reduce Salmonella adhesion to both cells (Caco-2 and HT-29), but only the CCMA 0743 and CCMA 0505 strains inhibited Escherichia coli adhesion to HT-29 cells in the competition assay. According to the results of these evaluated attributes, this strain showed to be an excellent candidate for probiotic use.-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer Nature-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceProbiotics and Antimicrobial Proteins-
Palavras-chave: dc.subjectAntagonistic effects-
Palavras-chave: dc.subjectBrazilian foods-
Palavras-chave: dc.subjectEnteropathogenic Escherichia coli-
Palavras-chave: dc.subjectEpithelial cells-
Palavras-chave: dc.subjectGastrointestinal tract-
Palavras-chave: dc.subjectSalmonella enteritidis-
Palavras-chave: dc.subjectLactobacilos - Propriedades probióticas-
Palavras-chave: dc.subjectBactérias enteropatogênicas-
Palavras-chave: dc.subjectCulinária brasileira-
Palavras-chave: dc.subjectCélulas epiteliais-
Palavras-chave: dc.subjectTrato gastrointestinal-
Título: dc.titleProbiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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