Polybia occidentalis and Polybia fastidiosa venom: a cytogenotoxic approach of effects on human and vegetal cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorPalmieri, Marcel José-
Autor(es): dc.creatorBarroso, Amanda Ribeiro-
Autor(es): dc.creatorAndrade-Vieira, Larissa Fonseca-
Autor(es): dc.creatorMonteiro, Marta Chagas-
Autor(es): dc.creatorSoares, Andreimar Martins-
Autor(es): dc.creatorCesar, Pedro Henrique Souza-
Autor(es): dc.creatorBraga, Mariana Aparecida-
Autor(es): dc.creatorTrento, Marcus Vinicius Cardoso-
Autor(es): dc.creatorMarcussi, Silvana-
Autor(es): dc.creatorDavide, Lisete Chamma-
Data de aceite: dc.date.accessioned2026-02-09T12:16:30Z-
Data de disponibilização: dc.date.available2026-02-09T12:16:30Z-
Data de envio: dc.date.issued2020-05-18-
Data de envio: dc.date.issued2020-05-18-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/41031-
Fonte completa do material: dc.identifierhttps://www.tandfonline.com/doi/abs/10.1080/01480545.2019.1631339?journalCode=idct20-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1158009-
Descrição: dc.descriptionThe venoms of wasps are a complex mixture of biologically active compounds, such as low molecular mass compounds, peptides, and proteins. The aim of the study was to evaluate the action of wasp venoms, Polybia occidentalis and Polybia fastidiosa, on the DNA of human leukocytes and on the cell cycle and genetic material of the plant model Lactuca sativa L. (lettuce). The cultured leukocytes were treated with the venoms and then evaluated by the comet assay. On another assay, seeds were exposed to a venom solution; the emitted roots were collected and the occurrence of cell cycle alterations (CCAs) and DNA fragmentation were evaluated by agarose gel electrophoresis and TUNEL assay. The results demonstrated that the venom of both wasps induces several CCAs and reduces the mitotic index (MI) on treated cells. They induced damage on human leukocytes DNA. High frequencies of fragments were observed in cells exposed to P. occidentalis venom, while those exposed to P. fastidiosa showed a high frequency of non-oriented chromosome. Both venoms induced the occurrence of various condensed nuclei (CN). This alteration is an excellent cytological mark to cell death (CD). Additionally, CD was evidenced by positive signals in TUNEL assay, by DNA fragmentation in agarose gel electrophoresis with vegetal cells, and by DNA fragmentation of the human leukocytes evaluated. Furthermore, human leukocytes exposed to the venom of P. fastidiosa had high rate of damage. The data demonstrate that both vegetal and human cells are adequate to evaluate the genotoxicity induced by venoms.-
Idioma: dc.languageen-
Publicador: dc.publisherTaylor and Francis Online-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceDrug and Chemical Toxicology-
Palavras-chave: dc.subjectCell cycle alterations-
Palavras-chave: dc.subjectTUNEL-
Palavras-chave: dc.subjectDNA fragmentation-
Palavras-chave: dc.subjectCell death-
Palavras-chave: dc.subjectHuman leukocytes-
Palavras-chave: dc.subjectComet assay-
Título: dc.titlePolybia occidentalis and Polybia fastidiosa venom: a cytogenotoxic approach of effects on human and vegetal cells-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

Não existem arquivos associados a este item.