A simple, inexpensive and successful freezing method for curimba prochilodus lineatus (characiformes) semen

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorViveiros, Ana Tereza de Mendonça-
Autor(es): dc.creatorOrfão, Laura Helena-
Autor(es): dc.creatorMaria, Alexandre Nizio-
Autor(es): dc.creatorAllaman, Ivan Bezerra-
Data de aceite: dc.date.accessioned2026-02-09T12:09:29Z-
Data de disponibilização: dc.date.available2026-02-09T12:09:29Z-
Data de envio: dc.date.issued2016-12-15-
Data de envio: dc.date.issued2016-12-15-
Data de envio: dc.date.issued2009-06-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/12071-
Fonte completa do material: dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0378432008001553-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1155565-
Descrição: dc.descriptionThe cryopreservation of fish sperm provides a tool by which reproduction is optimized and thereby larval production is increased. The aims of this study were to evaluate the effects of cryosolutions, motility-activation media, straw volumes and thawing temperatures on the post-thaw motility of curimba semen. Furthermore, semen cryopreserved in a simple and inexpensive cryosolution and that yielded excellent post-thaw motility was tested for fertility. Semen was diluted in each of the eight cryosolutions in a factorial of two cryoprotectants (DMSO and methylglycol) × four extenders (0.9% NaCl, 5% glucose, BTS™ and M III™). Diluted semen was frozen in 0.5-mL straws in a nitrogen vapor vessel. Sperm motility was evaluated after thawing (60 °C water bath for 8 s) and activation with a total of four different activation media (distilled water, 0.15% NaCl, 0.29% NaCl or 1% NaHCO3). To evaluate straw volume and thawing temperature, semen was diluted in 5% glucose and methylglycol and frozen in 0.5- and 4.0-mL straws. Half of the 0.5-mL straws were thawed in a water bath at 60 °C for 8 s and the other half at 30 °C for 16 s. The 4.0-mL straws were thawed at 60 °C for 24 s only. In the last experiment, semen cryopreserved in 5% glucose and methylglycol, 0.5-mL straws, and thawed at 60 °C for 8 s was tested for fertility. The results of these comparisons are presented and show that curimba semen can be successfully cryopreserved in a simple glucose solution combined with methylglycol as cryoprotectant, in 0.5-mL straws, yielding motility rates between 86% and 95% and fertilization rates between 47% and 83%.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceAnimal Reproduction Science-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectProchilodus lineatus-
Palavras-chave: dc.subjectSemen-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectFertilization-
Palavras-chave: dc.subjectSperm motility-
Título: dc.titleA simple, inexpensive and successful freezing method for curimba prochilodus lineatus (characiformes) semen-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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