Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients’ B cells

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Autor(es): dc.creatorCao, Yunlong-
Autor(es): dc.creatorSu, Bin-
Autor(es): dc.creatorGuo, Xianghua-
Autor(es): dc.creatorSun, Wenjie-
Autor(es): dc.creatorDeng, Yongqiang-
Autor(es): dc.creatorBao, Linlin-
Autor(es): dc.creatorZhu, Qinyu-
Autor(es): dc.creatorZhang, Xu-
Autor(es): dc.creatorZheng, Yinghui-
Autor(es): dc.creatorGeng, Chenyang-
Autor(es): dc.creatorChai, Xiaoran-
Autor(es): dc.creatorHe, Runsheng-
Autor(es): dc.creatorLi, Xiaofeng-
Autor(es): dc.creatorLv, Qi-
Autor(es): dc.creatorZhu, Hua-
Autor(es): dc.creatorDeng, Wei-
Autor(es): dc.creatorXu, Yanfeng-
Autor(es): dc.creatorWang, Yanjun-
Autor(es): dc.creatorQiao, Luxin-
Autor(es): dc.creatorTan, Yafang-
Autor(es): dc.creatorSong, Liyang-
Autor(es): dc.creatorWang, Guopeng-
Autor(es): dc.creatorDu, Xiaoxia-
Autor(es): dc.creatorGao, Ning-
Autor(es): dc.creatorLiu, Jiangning-
Autor(es): dc.creatorXiao, Junyu-
Autor(es): dc.creatorSu, Xiao-dong-
Autor(es): dc.creatorDu, Zongmin-
Autor(es): dc.creatorFeng, Yingmei-
Autor(es): dc.creatorQin, Chuan-
Autor(es): dc.creatorQin, Chengfeng-
Autor(es): dc.creatorJin, Ronghua-
Autor(es): dc.creatorXie, X. Sunney-
Data de aceite: dc.date.accessioned2026-02-09T12:05:31Z-
Data de disponibilização: dc.date.available2026-02-09T12:05:31Z-
Data de envio: dc.date.issued2020-08-03-
Data de envio: dc.date.issued2020-08-03-
Data de envio: dc.date.issued2020-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/42180-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0092867420306206-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1154123-
Descrição: dc.descriptionThe COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 14 potent neutralizing antibodies were identified, with the most potent one, BD-368-2, exhibiting an IC50 of 1.2 and 15 ng/mL against pseudotyped and authentic SARS-CoV-2, respectively. BD-368-2 also displayed strong therapeutic and prophylactic efficacy in SARS-CoV-2-infected hACE2-transgenic mice. Additionally, the 3.8 Å cryo-EM structure of a neutralizing antibody in complex with the spike-ectodomain trimer revealed the antibody’s epitope overlaps with the ACE2 binding site. Moreover, we demonstrated that SARS-CoV-2-neutralizing antibodies could be directly selected based on similarities of their predicted CDR3H structures to those of SARS-CoV-neutralizing antibodies. Altogether, we showed that human neutralizing antibodies could be efficiently discovered by high-throughput single B cell sequencing in response to pandemic infectious diseases.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceCell-
Palavras-chave: dc.subjectSingle-cell sequencing-
Palavras-chave: dc.subjectSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectNeutralizing antibody-
Palavras-chave: dc.subjectConvalescent patient-
Palavras-chave: dc.subjectB cell-
Título: dc.titlePotent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients’ B cells-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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