Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorAscari, I. J.-
Autor(es): dc.creatorAlves, N. G.-
Autor(es): dc.creatorJasmin, J.-
Autor(es): dc.creatorLima, R. R.-
Autor(es): dc.creatorQuintão, C. C. R.-
Autor(es): dc.creatorOberlender, G.-
Autor(es): dc.creatorMoraes, E. A.-
Autor(es): dc.creatorCamargo, L. S. A.-
Data de aceite: dc.date.accessioned2026-02-09T12:05:31Z-
Data de disponibilização: dc.date.available2026-02-09T12:05:31Z-
Data de envio: dc.date.issued2019-08-21-
Data de envio: dc.date.issued2019-08-21-
Data de envio: dc.date.issued2017-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/36381-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0739724017300589?via%3Dihub#!-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1154121-
Descrição: dc.descriptionThis study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5°C, or heat shock: 12 h at 41°C followed by 12 h at 38.5°C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM medium with IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceDomestic Animal Endocrinology-
Palavras-chave: dc.subjectThermoprotection-
Palavras-chave: dc.subjectHeat stress-
Palavras-chave: dc.subjectDNA fragmentation-
Palavras-chave: dc.subjectMitochondrial membrane potential-
Palavras-chave: dc.subjectROS production-
Palavras-chave: dc.subjectTermoproteção-
Palavras-chave: dc.subjectEstresse por calor-
Palavras-chave: dc.subjectFragmentação de DNA-
Palavras-chave: dc.subjectPotencial de membrana mitocondrial-
Palavras-chave: dc.subjectProdução de ROS-
Título: dc.titleAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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