Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorSilva, D. M.-
Autor(es): dc.creatorZangeronimo, M. G.-
Autor(es): dc.creatorMurgas, L. D. S.-
Autor(es): dc.creatorRocha, L. G. P.-
Autor(es): dc.creatorChaves, B. R.-
Autor(es): dc.creatorPereira, B. A.-
Autor(es): dc.creatorCunha, E. C. P.-
Data de aceite: dc.date.accessioned2026-02-09T12:04:01Z-
Data de disponibilização: dc.date.available2026-02-09T12:04:01Z-
Data de envio: dc.date.issued2020-03-05-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2020-03-05-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2011-12-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57533-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1096637411000980?via%3Dihub#!-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1153588-
Descrição: dc.descriptionObjective To evaluate in vitro IGF-I treatment during warming of storage-cooled boar semen and its effect on seminal quality parameters and metabolism in spermatic cells. Design Semen samples (n = 7) warmed after stored at 15 °C for 24 or 72 h were divided into four equal parts. Different IGF-I concentrations (0, 50, 100 and 150 ng/mL) were added to the semen samples. The samples were incubated at 37 °C, and assessments were made after 0 and 120 min of incubation. Results For semen samples that were stored for 24 h, the addition of IGF-I had no effect (p > 0.05) on the total motility and intensity of movements by spermatic cells, osmotic resistance, live:dead cell ratio or total spermatic abnormalities. However, incubation with 150 ng/mL IGF-I did decrease glutathione peroxidase activity (p < 0.05) and reduce lipid peroxidation after 120 min of incubation. For semen samples stored for 72 h and incubated with IGF-I for 120 min, there was a linear relationship between the IGF-I concentration and the live:dead ratio (p < 0.05). There was a quadratic relationship between the IGF-I concentration and both the osmotic resistance (peak results at IGF-I = 62.4 ng/mL) and glutathione peroxidase activity (peak results at IGF-I = 77.8 ng/mL). There was no effect on lipid peroxidation (p > 0.05) after 120 min of incubation. Addition of IGF-I also decreased fructose utilization by spermatic cells regardless of semen storage time (p < 0.05). Conclusion This study suggests that IGF-I may be beneficial to semen stored for longer periods of time. Adding 150 ng/mL IGF-I improved the quality of semen stored for 24 h, and adding 78 ng/mL IGF-I improved the quality of semen stored for 72 h.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceGrowth Hormone & IGF Research-
Palavras-chave: dc.subjectInsulin-like growth factor I-
Palavras-chave: dc.subjectBoar spermatozoa-
Palavras-chave: dc.subjectMotility-
Palavras-chave: dc.subjectFunctional membrane integrity-
Palavras-chave: dc.subjectLipid peroxidation-
Palavras-chave: dc.subjectFructose uptake-
Palavras-chave: dc.subjectSuíno - Espermatozoide-
Palavras-chave: dc.subjectSêmen - Resfriamento-
Palavras-chave: dc.subjectEsperma - Qualidade-
Título: dc.titleAddition of IGF-I to storage-cooled boar semen and its effect on sperm quality-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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