Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorBastos, Sabrina Carvalho-
Autor(es): dc.creatorPimenta, Carlos José-
Autor(es): dc.creatorDias, Disney Ribeiro-
Autor(es): dc.creatorChalfoun, Sara Maria-
Autor(es): dc.creatorAngélico, Caroline Lima-
Autor(es): dc.creatorTavares, Lucas Silva-
Data de aceite: dc.date.accessioned2026-02-09T11:57:14Z-
Data de disponibilização: dc.date.available2026-02-09T11:57:14Z-
Data de envio: dc.date.issued2014-10-27-
Data de envio: dc.date.issued2014-10-27-
Data de envio: dc.date.issued2013-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/4487-
Fonte completa do material: dc.identifierhttp://www.idosi.org/wjas/wjas9(2)13/9.pdf-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1151123-
Descrição: dc.descriptionTo examine tThe optimal conditions of cultivation for Cladosporium cladosporioides to produce and partially purify pectin methylesterase (PME) and polygalacturonase (PG) were examined under laboratory conditions . Activities of PME and PG were evaluated after 10, 15 and 20 days of incubation at 28°C in rice solid medium. Enzyme extraction was carried out by homogenization in buffer solutions at different pH (4, 5 and 6) followed by precipitation with ammonium sulfate [(NH4)2SO4] at 20, 40 and 60% saturation. After 10 days of incubation, the highest activities for PG (105.5 U/mL) and PME (1480 U/mL) in the crude extract was observed. Benzoate buffer (pH 4.0) was the best buffer for enzymes extraction. The final yield of 108.7% for PME and 10.6% for PG and a purification index of 14.2 and 1.4 for PME and PG, respectively, was obtained after saturation with 60%. The ideal conditions for obtained and pre-purification of PME and PG were 10 days of incubation, extraction with benzoate buffer pH 4.0 and precipitation with 60% of (NH4)2SO4. This is the first report on of pectinases pectinolytic enzymes produced by from C. cladosporioides and provides information about growth conditions for production and pre-purification of PME and PG.-
Idioma: dc.languageen-
Publicador: dc.publisherWorld Science Research Journals (WSRJ)-
Direitos: dc.rightsrestrito-
???dc.source???: dc.sourceWorld Journal of Agricultural Sciences (Print)-
Palavras-chave: dc.subjectPectinolytic enzymes-
Palavras-chave: dc.subjectFilamentous fungi-
Palavras-chave: dc.subjectPectin-
Palavras-chave: dc.subjectCoffee fermentation-
Título: dc.titlePectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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