Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semen

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorResende, Carla Oliveira-
Autor(es): dc.creatorBetarelli, Rafael Pedroso-
Autor(es): dc.creatorRabelo, Stenia Severo-
Autor(es): dc.creatorChaves, Bruna Resende-
Autor(es): dc.creatorRodriguez-Gil, Joan Enric-
Autor(es): dc.creatorZangeronimo, Marcio Gilberto-
Data de aceite: dc.date.accessioned2026-02-09T11:48:28Z-
Data de disponibilização: dc.date.available2026-02-09T11:48:28Z-
Data de envio: dc.date.issued2020-05-15-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2020-05-15-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2019-09-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57459-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S037843201930185X?via%3Dihub#!-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1147894-
Descrição: dc.descriptionThe objective of this study was to evaluate the effect of adding reduced glutathione (GSH) to a boar semen freezing extender supplemented with insulin-like growth factor I (IGF-I) or anti-IGF-I. Eight ejaculates from eight boars were extended to obtain insemination doses, which were supplemented with either recombinant human IGF-I (30 ng/mL) or anti-IGF-I (60 ng/mL) shortly after extension. After 24 h of liquid storage at 17 °C, the semen was frozen with or without GSH (5 mM) in the freezing extender for a total of six treatments. Osmotic resistance and acrosome integrity was greater in fresh semen (P <  0.05) soon after adding IGF-I or the anti-IGF-I antibody. After 24 h of cooling, the supplementation with these compounds resulted in an increased (P <  0.05) percentage of sperm with relatively greater mitochondrial activity and reduced the percentage of cells with relatively greater concentrations of superoxide. After thawing, there was a reduction (P <  0.05) in the percentage and fluorescence intensity of sperm with greater quantities of superoxide and peroxide only in samples treated with GSH + IGF-I and GSH + anti-IGF-I. The addition of GSH (alone or in combination with IGF-I or anti-IGF-I), however, reduced the percentage of sperm with an intact acrosome (P < 0.05). The same effect was not observed with IGF-I or anti-IGF-I alone. In conclusion, the addition of IGF-I or anti-IGF-I improved the quality of fresh or liquid-stored semen. Using GSH in the freezing extender improved the antioxidant potential of frozen semen only in combination with IGF-I or an anti-IGF-I antibody.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceAnimal Reproduction Science-
Palavras-chave: dc.subjectAntioxidant-
Palavras-chave: dc.subjectBoar spermatozoa-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectLipid peroxidation-
Palavras-chave: dc.subjectSemen-
Palavras-chave: dc.subjectThawed semen-
Palavras-chave: dc.subjectAntioxidante-
Palavras-chave: dc.subjectSuíno - Espermatozóides-
Palavras-chave: dc.subjectCriopreservação-
Palavras-chave: dc.subjectPeroxidação lipídica-
Palavras-chave: dc.subjectSêmen-
Palavras-chave: dc.subjectSêmen descongelado-
Título: dc.titleAddition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semen-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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