Neutrophil-generated HOCL leads to non-specific thiol oxidation in phagocytized bacteria

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorDegrossoli, Adriana-
Autor(es): dc.creatorMüller, Alexandra-
Autor(es): dc.creatorXie, Kaibo-
Autor(es): dc.creatorSchneider, Jannis F.-
Autor(es): dc.creatorBader, Verian-
Autor(es): dc.creatorWinklhofer, Konstanze F.-
Autor(es): dc.creatorMeyer, Andreas J.-
Autor(es): dc.creatorLeichert, Lars I.-
Data de aceite: dc.date.accessioned2026-02-09T11:36:57Z-
Data de disponibilização: dc.date.available2026-02-09T11:36:57Z-
Data de envio: dc.date.issued2019-02-19-
Data de envio: dc.date.issued2019-02-19-
Data de envio: dc.date.issued2018-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/32848-
Fonte completa do material: dc.identifierhttps://elifesciences.org/articles/32288-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1143581-
Descrição: dc.descriptionPhagocytic immune cells kill pathogens in the phagolysosomal compartment with a cocktail of antimicrobial agents. Chief among them are reactive species produced in the so-called oxidative burst. Here, we show that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols. Using roGFP2-based fusion probes, we could show that this massive breakdown of the thiol redox homeostasis was dependent on phagocytosis, presence of NADPH oxidase and ultimately myeloperoxidase. Interestingly, the redox-mediated fluorescence change in bacteria expressing a glutathione-specific Grx1-roGFP2 fusion protein or an unfused roGFP2 showed highly similar reaction kinetics to the ones observed with roGFP2-Orp1, under all conditions tested. We recently observed such an indiscriminate oxidation of roGFP2-based fusion probes by HOCl with fast kinetics in vitro. In line with these observations, abating HOCl production in immune cells with a myeloperoxidase inhibitor significantly attenuated the oxidation of all three probes in bacteria.-
Idioma: dc.languageen-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceeLife-
Título: dc.titleNeutrophil-generated HOCL leads to non-specific thiol oxidation in phagocytized bacteria-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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