Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorArmesto, Cecilia-
Autor(es): dc.creatorMaia, Fernanda Gonçalves Martins-
Autor(es): dc.creatorAbreu, Mário Sobral de-
Autor(es): dc.creatorFigueira, Antonia dos Reis-
Autor(es): dc.creatorSilva, Bruno Marques da-
Autor(es): dc.creatorMonteiro, Fernando Pereira-
Data de aceite: dc.date.accessioned2026-02-09T11:35:11Z-
Data de disponibilização: dc.date.available2026-02-09T11:35:11Z-
Data de envio: dc.date.issued2017-03-24-
Data de envio: dc.date.issued2017-03-24-
Data de envio: dc.date.issued2012-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/12556-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1142934-
Descrição: dc.descriptionBlister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherSociedade Brasileira de Microbiologia-
Direitos: dc.rightsacesso aberto-
???dc.source???: dc.sourceBrazilian Journal of Microbiology-
Palavras-chave: dc.subjectColletotrichum gloeosporioides-
Palavras-chave: dc.subjectFluorescent proteins-
Palavras-chave: dc.subjectPathogenicity-
Título: dc.titleGenetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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