Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorSilva, Viviam de Oliveira-
Autor(es): dc.creatorPereira, Luciano José-
Autor(es): dc.creatorMurata, Ramiro Mendonça-
Data de aceite: dc.date.accessioned2026-02-09T11:34:57Z-
Data de disponibilização: dc.date.available2026-02-09T11:34:57Z-
Data de envio: dc.date.issued2018-11-23-
Data de envio: dc.date.issued2018-11-23-
Data de envio: dc.date.issued2017-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/31918-
Fonte completa do material: dc.identifierhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0946-1-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1142856-
Descrição: dc.descriptionBackground: The aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant. Results: The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan. Conclusions: Treatment with β-glucans positively modulated the immune response and production of metabolites.-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer Nature-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceBMC Microbiology-
Palavras-chave: dc.subjectAggregatibacter actinomycetemcomitans-
Palavras-chave: dc.subjectPeriodontal disease-
Palavras-chave: dc.subjectHost response-
Palavras-chave: dc.subjectKeratinocyte-
Palavras-chave: dc.subjectFibroblast-
Palavras-chave: dc.subjectImmunomodulation-
Palavras-chave: dc.subjectDoença periodontal-
Palavras-chave: dc.subjectResposta do hospedeiro-
Palavras-chave: dc.subjectQueratinócito-
Palavras-chave: dc.subjectFibroblasto-
Título: dc.titleOral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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