Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit RPB1

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorBadjatia, Nitika-
Autor(es): dc.creatorAmbrósio, Daniela L.-
Autor(es): dc.creatorLee, Ju Huck-
Autor(es): dc.creatorGünzl, Arthur-
Data de aceite: dc.date.accessioned2026-02-09T11:34:37Z-
Data de disponibilização: dc.date.available2026-02-09T11:34:37Z-
Data de envio: dc.date.issued2017-09-04-
Data de envio: dc.date.issued2017-09-04-
Data de envio: dc.date.issued2013-05-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/15355-
Fonte completa do material: dc.identifierhttp://mcb.asm.org/content/33/10/1965.full.pdf+html-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1142732-
Descrição: dc.descriptionConserved from yeast to mammals, phosphorylation of the heptad repeat sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 in the carboxy-terminal domain (CTD) of the largest RNA polymerase II (RNA Pol II) subunit, RPB1, mediates the enzyme's promoter escape and binding of RNA-processing factors, such as the m7G capping enzymes. The first critical step, Ser5 phosphorylation, is carried out by cyclin-dependent kinase 7 (CDK7), a subunit of the basal transcription factor TFIIH. Many early-diverged protists, such as the lethal human parasite Trypanosoma brucei, however, lack the heptad repeats and, apparently, a CDK7 ortholog. Accordingly, characterization of trypanosome TFIIH did not identify a kinase component. The T. brucei CTD, however, is phosphorylated and essential for transcription. Here we show that silencing the expression of T. brucei cdc2-related kinase 9 (CRK9) leads to a loss of RPB1 phosphorylation. Surprisingly, this event did not impair RNA Pol II transcription or cotranscriptional m7G capping. Instead, we observed that CRK9 silencing led to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA maturation, that was caused by hypomethylation of the SL RNA's unique cap4.-
Descrição: dc.descriptionhttps://repositorio.ufla.br/handle/1/15355-
Idioma: dc.languageen-
Publicador: dc.publisherNational Institutes of Health-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceMollecular and Cellular Biology-
Palavras-chave: dc.subjectRNA polymerase-
Palavras-chave: dc.subjectMethylation-
Palavras-chave: dc.subjectPhosphorylation-
Palavras-chave: dc.subjectCarboxy-terminal domain-
Palavras-chave: dc.subjectMetilação-
Palavras-chave: dc.subjectFosforilação-
Palavras-chave: dc.subjectDomínio carboxi-terminal-
Título: dc.titleTrypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit RPB1-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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