Preparative Separation and Structural Identification of Impurities of a New α2-Adrenoceptor Agonist Using Stacking Injection, LC-MSn and LC-SPE-NMR

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorCardoso, Josiane O.-
Autor(es): dc.creatorThomasi, Sérgio S.-
Autor(es): dc.creatorVenâncio, Tiago-
Autor(es): dc.creatorPitta, Ivan R.-
Autor(es): dc.creatorLima, Maria do Carmo A. de-
Autor(es): dc.creatorOliveira, Regina V.-
Data de aceite: dc.date.accessioned2026-02-09T11:33:44Z-
Data de disponibilização: dc.date.available2026-02-09T11:33:44Z-
Data de envio: dc.date.issued2019-01-25-
Data de envio: dc.date.issued2019-01-25-
Data de envio: dc.date.issued2017-06-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/32516-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1142418-
Descrição: dc.descriptionIdentifying impurities in drug substances has become one of the most important issues in pharmaceutical analysis since it can have a significant impact on the efficacy of new pharmaceutical products. Due to the purity requirements, in this paper a new synthetic α2-adrenoceptor agonist, called LPSF-PT-31, was purified and its impurities were characterized by liquid chromatography-multistage mass spectrometry (LC-MSn) and liquid chromatography-solid phase extraction-nuclear magnetic resonance (LC-SPE-NMR). The purification step was conducted using a semi-preparative liquid chromatography and stacked injections as a new approach to drug purification. As a result, a total yield of 75% of the pure LPSF-PT-31 and 2.9 L day-1 in solvent reduction was obtained. The combination of semi-preparative stacking injection, LC-MSn, and LC-SPE-NMR, demonstrated to be efficient to purify active drugs and unambiguously identify its impurities. In addition, isolation and identification of drug impurities in the early stages of development can improve the synthetic pathway, preventing the formation of impurities or minimizing this formation to minimum levels.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherSociedade Brasileira de Química-
Direitos: dc.rightsacesso aberto-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
???dc.source???: dc.sourceJournal of the Brazilian Chemical Society-
Palavras-chave: dc.subjectSemi-preparative separation-
Palavras-chave: dc.subjectStacked injection-
Palavras-chave: dc.subjectImpurity identification-
Palavras-chave: dc.subjectSeparação semi-preparativa-
Palavras-chave: dc.subjectInjeção empilhada-
Palavras-chave: dc.subjectIdentificação de impurezas-
Título: dc.titlePreparative Separation and Structural Identification of Impurities of a New α2-Adrenoceptor Agonist Using Stacking Injection, LC-MSn and LC-SPE-NMR-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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