The protective effect exerted by ascorbic acid on DNA fragmentation of human leukocytes induced by Lachesis muta muta venom

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorTrento, Marcus Vinícius Cardoso-
Autor(es): dc.creatorEleutério, Mateus William de Faria-
Autor(es): dc.creatorAbreu, Tatiane Silva-
Autor(es): dc.creatorMachado, Gustavo Henrique Andrade-
Autor(es): dc.creatorCesar, Pedro Henrique Souza-
Autor(es): dc.creatorSimão, Anderson Assaid-
Autor(es): dc.creatorMarcussi, Silvana-
Data de aceite: dc.date.accessioned2026-02-09T11:29:43Z-
Data de disponibilização: dc.date.available2026-02-09T11:29:43Z-
Data de envio: dc.date.issued2019-06-13-
Data de envio: dc.date.issued2019-06-13-
Data de envio: dc.date.issued2019-03-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/34761-
Fonte completa do material: dc.identifierhttps://onlinelibrary.wiley.com/doi/10.1002/jcb.27628-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1141895-
Descrição: dc.descriptionThe objective of this study was to evaluate the genotoxic and mutagenic effects of the toxins present in Lachesis muta muta's venom on human peripheral blood leukocytes and the protective potential of ascorbic acid on DNA fragmentation. The venom of L. muta muta was incubated in different concentrations (1, 2.5, 5, 7.5, 10, 15, 20, 30, 40, 50, 60, and 120 µg/mL) with human blood to evaluate DNA fragmentation using the comet, agarose gel electrophoresis, and micronucleus assays. In these concentrations evaluated, the venom of L. muta muta induced genotoxicity (comet assay and agarose gel electrophoresis) and mutagenicity (micronucleus test), but they were not cytotoxic, as they did not change the rate of cell proliferation after cytokinesis blockade with cytochalasin B. The ascorbic acid significantly inhibited the genotoxicity induced by L. muta muta venom in the proportions evaluated (1:0.1 and 1:0.5, venom/ascorbic acid ‐ w/w). Thus, future studies are needed to elucidate the protective mechanisms of ascorbic acid on the genotoxic effects induced by toxins present in snake venoms.-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceJournal of Cellular Biochemistry-
Palavras-chave: dc.subjectComet assay-
Palavras-chave: dc.subjectGenotoxicity-
Palavras-chave: dc.subjectMicronucleus-
Palavras-chave: dc.subjectSnake venom-
Título: dc.titleThe protective effect exerted by ascorbic acid on DNA fragmentation of human leukocytes induced by Lachesis muta muta venom-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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