Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorOliveira, V. M.-
Autor(es): dc.creatorMansanares, M. E.-
Autor(es): dc.creatorSemir, J.-
Autor(es): dc.creatorForni-Martins, E. R.-
Data de aceite: dc.date.accessioned2026-02-09T11:28:35Z-
Data de disponibilização: dc.date.available2026-02-09T11:28:35Z-
Data de envio: dc.date.issued2020-12-01-
Data de envio: dc.date.issued2020-12-01-
Data de envio: dc.date.issued2012-12-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/45728-
Fonte completa do material: dc.identifierhttps://www.geneticsmr.com/articles/1879-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1141574-
Descrição: dc.descriptionIn order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes.-
Idioma: dc.languageen-
Publicador: dc.publisherFundação de Pesquisas Científicas de Ribeirão Preto – FUNPEC-RP-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceGenetics and Molecular Research-
Palavras-chave: dc.subjectAgNOR banding-
Palavras-chave: dc.subjectCMA banding-
Palavras-chave: dc.subjectPhysical mapping-
Palavras-chave: dc.subject45S rDNA site-
Título: dc.titleKaryotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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