Experimental periodontal disease triggers coronary endothelial dysfunction in middle-aged rats: preventive effect of a prebiotic β-glucan

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorSilva, Grazielle Caroline-
Autor(es): dc.creatorCosta, Eduardo Damasceno-
Autor(es): dc.creatorLemos, Virgínia Soares-
Autor(es): dc.creatorQueiroz-Junior, Celso Martins-
Autor(es): dc.creatorPereira, Luciano José-
Data de aceite: dc.date.accessioned2026-02-09T11:27:57Z-
Data de disponibilização: dc.date.available2026-02-09T11:27:57Z-
Data de envio: dc.date.issued2022-05-30-
Data de envio: dc.date.issued2022-05-30-
Data de envio: dc.date.issued2021-03-03-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/50056-
Fonte completa do material: dc.identifierhttps://academic.oup.com/biomedgerontology/article/76/8/1398/6157087-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1141361-
Descrição: dc.descriptionThis study was aimed to verify the hypothesis that periodontal disease contributes to endothelial dysfunction in the coronary arteries of middle-aged rats. Besides we evaluated the effects of a prebiotic (β-glucan isolated from Saccharomyces cerevisiae) in preventing vascular dysfunction. The sample comprised young (sham and induced to periodontal disease) and middle-aged rats (sham, periodontal disease, sham-treated and periodontal disease-treated), at 12 and 57 weeks, respectively. The treated-groups received daily doses of β-glucan (50 mg/kg) orally (gavage) for 4 weeks, and periodontal disease was induced in the last 2 weeks by ligature. A myograph system assessed vascular reactivity. The expression of endothelial nitric oxide synthase (eNOS), cyclooxygenase 1 (COX-1), COX-2, p47phox, gp91phox, NF-KB p65, p53, p21, and p16 was quantified by western blotting. Serum hydroperoxide production was measured by the ferrous oxidation–xylenol orange (FOX-2) assay method. Interleukin-1 beta (IL-1β), IL-10, and tumor necrosis factor-alpha (TNF-α) levels were evaluated by spectroscopic ultraviolet-visible analysis. Periodontal disease in middle-aged rats was associated with reduced acetylcholine-induced relaxations of coronary artery rings affecting the endothelium-dependent hyperpolarization- and the nitric oxide-mediated relaxations. The endothelial dysfunction was related to eNOS downregulation, pronounced impairment of the EDH-mediated relaxation, increased IL-1β and TNF-α proinflammatory cytokines, and also upregulation of NADPH oxidase and COXs, starting accumulate aging markers such as p53/p21 and the p16. Treatment with β-glucan effectively reduced bone loss in periodontal disease and delayed endothelial dysfunction in the coronary artery. Our data show that yeast β-glucan ingestion prevented oxidative stress and synthesis of proinflammatory marker and prevented eNOS reduction induced by periodontal disease in middle-aged rats. These results suggest that β-glucan has a beneficial effect on the coronary vascular bed.-
Idioma: dc.languageen-
Publicador: dc.publisherOxford University Press (OUP)-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceJournals of Gerontology Series A-Biological Sciences and Medical Sciences-
Palavras-chave: dc.subjectβ-Glucan-
Palavras-chave: dc.subjectCoronary reactivity-
Palavras-chave: dc.subjectEndothelium-
Palavras-chave: dc.subjectPeriodontal disease-
Título: dc.titleExperimental periodontal disease triggers coronary endothelial dysfunction in middle-aged rats: preventive effect of a prebiotic β-glucan-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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