Prevalence of SARS-CoV-2 in human post-mortem ocular tissues

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorSawant, Onkar B.-
Autor(es): dc.creatorSingh, Sneha-
Autor(es): dc.creatorWright III, Robert Emery-
Autor(es): dc.creatorJones, Kayla M.-
Autor(es): dc.creatorTitus, Michael S.-
Autor(es): dc.creatorDennis, Eugene-
Autor(es): dc.creatorHicks, Eric-
Autor(es): dc.creatorMajmudar, Parag A.-
Autor(es): dc.creatorKumar, Ashok-
Autor(es): dc.creatorMian, Shahzad I.-
Data de aceite: dc.date.accessioned2026-02-09T11:26:50Z-
Data de disponibilização: dc.date.available2026-02-09T11:26:50Z-
Data de envio: dc.date.issued2020-11-08-
Data de envio: dc.date.issued2020-11-08-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/45423-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1542012420301683#!-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1140970-
Descrição: dc.descriptionBackground SARS-CoV-2 is found in conjunctival swabs and tears of COVID-19 patients. However, the presence of SARS-CoV-2 has not been detected in the human eye to date. We undertook this study to analyze the prevalence of SARS-CoV-2 in human post-mortem ocular tissues. Methods The expression of SARS-CoV-2 RNA was assessed by RT-PCR in corneal and scleral tissues from 33 surgical-intended donors who were eliminated from a surgical use per Eye Bank Association of America (EBAA) donor screening guidelines or medical director review or positive COVID-19 test. Ocular levels of SARS-CoV-2 RNA (RT-PCR), Envelope and Spike proteins (immunohistochemistry) and anti-SARS-CoV-2 IgG and IgM antibodies (ELISA) in blood were evaluated in 10 COVID-19 donors. Findings Of 132 ocular tissues from 33 surgical-intended donors, the positivity rate for SARS-CoV-2 RNA was ∼13% (17/132). Of 10 COVID-19 donors, six had PCR positive post-mortem nasopharyngeal swabs whereas eight exhibited positive post-mortem anti-SARS-CoV-2 IgG levels. Among 20 eyes recovered from 10 COVID-19 donors: three conjunctival, one anterior corneal, five posterior corneal, and three vitreous swabs tested positive for SARS-CoV-2 RNA. SARS-CoV-2 spike and envelope proteins were detected in epithelial layer of the corneas that were procured without Povidone-Iodine (PVP–I) disinfection. Interpretations Our study showed a small but noteworthy prevalence of SARS-CoV-2 in ocular tissues from COVID-19 donors. These findings underscore the criticality of donor screening guidelines, post-mortem nasopharyngeal PCR testing and PVP-I disinfection protocol to eliminate any tissue harboring SARS-CoV-2 being used for corneal transplantation.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceThe Ocular Surface-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectCoronavirus-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectCorneal transplantation-
Palavras-chave: dc.subjectTransplante de córnea-
Título: dc.titlePrevalence of SARS-CoV-2 in human post-mortem ocular tissues-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

Não existem arquivos associados a este item.