Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorDorneles, Elaine M. S.-
Autor(es): dc.creatorSantana, Jordana A.-
Autor(es): dc.creatorRibeiro, Dayana-
Autor(es): dc.creatorDorella, Fernanda Alves-
Autor(es): dc.creatorGuimarães, Alessandro S.-
Autor(es): dc.creatorMoawad, Mohamed S.-
Autor(es): dc.creatorSelim, Salah A.-
Autor(es): dc.creatorGaraldi, Ana Luiza M.-
Autor(es): dc.creatorMiyoshi, Anderson-
Autor(es): dc.creatorRibeiro, Márcio G.-
Autor(es): dc.creatorGouveia, Aurora M. G.-
Autor(es): dc.creatorAzevedo, Vasco-
Autor(es): dc.creatorHeinemann, Marcos B.-
Autor(es): dc.creatorLage, Andrey P.-
Data de aceite: dc.date.accessioned2026-02-09T11:26:27Z-
Data de disponibilização: dc.date.available2026-02-09T11:26:27Z-
Data de envio: dc.date.issued2018-02-23-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2018-02-23-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2014-06-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57105-
Fonte completa do material: dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098758-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1140848-
Descrição: dc.descriptionThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.-
Idioma: dc.languageen-
Publicador: dc.publisherPLoS ONE-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourcePLoS ONE-
Palavras-chave: dc.subjectCorynebacterium pseudotuberculosis-
Palavras-chave: dc.subjectEnterobacterial repetitive intergenic consensus-
Palavras-chave: dc.subjectMolecular epidemiology investigations-
Palavras-chave: dc.subjectConsenso intergênico repetitivo enterobactério-
Palavras-chave: dc.subjectInvestigações epidemiológicas moleculares-
Título: dc.titleEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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