In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorCampos, J. M. S.-
Autor(es): dc.creatorDavide, L. C.-
Autor(es): dc.creatorSalgado, C. C.-
Autor(es): dc.creatorSantos, F. C.-
Autor(es): dc.creatorCosta, P. N.-
Autor(es): dc.creatorSilva, P. S.-
Autor(es): dc.creatorAlves, C. C. S.-
Autor(es): dc.creatorViccini, L. F.-
Autor(es): dc.creatorPereira, A. V.-
Data de aceite: dc.date.accessioned2026-02-09T11:20:13Z-
Data de disponibilização: dc.date.available2026-02-09T11:20:13Z-
Data de envio: dc.date.issued2020-02-11-
Data de envio: dc.date.issued2020-02-11-
Data de envio: dc.date.issued2009-02-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/39004-
Fonte completa do material: dc.identifierhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0523.2008.01546.x-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1138725-
Descrição: dc.descriptionThis study describes an efficient in vitro method using colchicine‐treated triploid seedlings of Napiergrass and Pearl millet hybrids to produce hexaploid hybrids and their subsequent identification with flow cytometry. It also describes chromosome count and stomatal morphology and their use to ploidy analysis. Four‐hundred and eighty triploid seeds, representing 12 different hybrids were sterilized and transferred to MS media to induce chromosome doubling. Surviving plants were analysed by flow cytometry. From six triploid (control) and hexaploid plants, the stomata sizes and frequency were analysed. Chromosome count was performed only in the plants identified as hexaploid. Seventeen plants were identified as hexaploid by flow cytometry analysis. Further confirmation of the hexaploid condition was performed with stomatal morphology (stomatal frequency reduction and stomatal length increase) and chromosome count (2n = 6x = 42). Chromosome doubling has numerous applications in Pennisetum breeding. It can be used to restore the fertility of interspecific hybrids and to improve seed size.-
Idioma: dc.languageen-
Publicador: dc.publisherBlackwell Verlag-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourcePlant Breeding-
Palavras-chave: dc.subjectChromosome doubling-
Palavras-chave: dc.subjectColchicine-
Palavras-chave: dc.subjectPennisetum hybrids-
Palavras-chave: dc.subjectFlow cytometry-
Palavras-chave: dc.subjectDuplicação cromossômica-
Palavras-chave: dc.subjectColchicina-
Palavras-chave: dc.subjectCitometria de fluxo-
Título: dc.titleIn vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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