Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorOliveira, Ana Cristina Freitas de-
Autor(es): dc.creatorNeves, Isabelle Cistina Oliveira-
Autor(es): dc.creatorSaraiva, Jamile Alves Meireles-
Autor(es): dc.creatorCarvalho, Marcelle Flores Fernandes de-
Autor(es): dc.creatorBatista, Gioanni Aleixo-
Autor(es): dc.creatorVeríssimo, Lizzy Ayra Alcântara-
Autor(es): dc.creatorResende, Jaime Vilela de-
Data de aceite: dc.date.accessioned2026-02-09T11:17:02Z-
Data de disponibilização: dc.date.available2026-02-09T11:17:02Z-
Data de envio: dc.date.issued2020-04-15-
Data de envio: dc.date.issued2020-04-15-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/40087-
Fonte completa do material: dc.identifierhttps://www.tandfonline.com/doi/abs/10.1080/01496395.2019.1617743?journalCode=lsst20-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1137555-
Descrição: dc.descriptionDifferent protocols for functionalization of cryogels with L-tryptophan were tested for the lysozyme adsorption. The effect of ionic strength and pH on the adsorptive capacity (q) was investigated. The morphological, thermal, and mechanical properties of the cryogels were determined. A maximum adsorption for lysozyme (58.030 mg g–1) was achieved when 5% (v/v) of glutaraldehyde (at pH 6.0) and no methanol were used during the cryogel functionalization. The hydrophobic cryogel presented a porosity of 0.666. A strong single band at 14.3 kDa was visualized in SDS-PAGE, indicating that the hydrophobic affinity cryogels was efficient for the lysozyme capture.-
Idioma: dc.languageen-
Publicador: dc.publisherTaylor & Francis-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceSeparation Science and Technology-
Palavras-chave: dc.subjectCryogels - Functionalization-
Palavras-chave: dc.subjectPreparative chromatography-
Palavras-chave: dc.subjectLysozyme adsorption-
Palavras-chave: dc.subjectHydrophobic affinity-
Palavras-chave: dc.subjectCriogéis - Funcionalização-
Palavras-chave: dc.subjectCromatografia preparativa-
Palavras-chave: dc.subjectAdsorção de lisozima-
Palavras-chave: dc.subjectAfinidade hidrofóbica-
Título: dc.titleCapture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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