Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorMoreira, Marcela L.-
Autor(es): dc.creatorDorneles, Elaine M. S.-
Autor(es): dc.creatorSoares, Rodrigo P.-
Autor(es): dc.creatorMagalhães, Camila P.-
Autor(es): dc.creatorCosta‑Pereira, Christiane-
Autor(es): dc.creatorLage, Andrey P.-
Autor(es): dc.creatorTeixeira‑Carvalho, Andréa-
Autor(es): dc.creatorMartins‑Filho, Olindo A.-
Autor(es): dc.creatorAraújo, Márcio S. S.-
Data de aceite: dc.date.accessioned2026-02-09T11:14:41Z-
Data de disponibilização: dc.date.available2026-02-09T11:14:41Z-
Data de envio: dc.date.issued2018-02-23-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2018-02-23-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2015-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57830-
Fonte completa do material: dc.identifierhttps://actavetscand.biomedcentral.com/articles/10.1186/s13028-015-0142-y-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1136718-
Descrição: dc.descriptionThe process for obtaining monoclonal antibodies against a specific antigen is very laborious, involves sophisticated technologies and it is not available in most research laboratories. Considering that most cytokines remain partially conserved among species during evolution, the search for antibody cross-reactivity is an important strategy for immunological studies in veterinary medicine. In this context, the amino acid sequence from human and canine cytokines have demonstrated 49–96 % homology, suggesting high probability of cross-reactivity amongst monoclonal antibodies. For this, 17 commercially available anti-human monoclonal antibodies [IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-10, IL-12, IL-13, IL-17A, IFN-γ (#1, #2), TNF-α (#1, #2) and TGF-β], were evaluated in vitro for intracellular cytokine detection in a stimulated canine blood culture by flow cytometry and confocal microscopy. Lymphocytes from peripheral blood of healthy and two unhealthy dogs were analyzed.-
Idioma: dc.languageen-
Publicador: dc.publisherBioMed Central-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceActa Veterinaria Scandinavica-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectMonoclonal antibodies-
Palavras-chave: dc.subjectCross-reactivity-
Palavras-chave: dc.subjectFlow cytometry-
Palavras-chave: dc.subjectCitocinas-
Palavras-chave: dc.subjectAnticorpos monoclonais-
Palavras-chave: dc.subjectReatividade cruzada-
Palavras-chave: dc.subjectCitometria de fluxo-
Título: dc.titleCross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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