Histone acetylation during the in vitro maturation of bovine oocytes with different levels of competence

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorPontelo, Thais Preisser-
Autor(es): dc.creatorRodrigues, Sarah de Andrade Dias-
Autor(es): dc.creatorKawamoto, Taynan Stonoga-
Autor(es): dc.creatorLeme, Ligiane de Oliveira-
Autor(es): dc.creatorGomes, A. C. M. M.-
Autor(es): dc.creatorZangeronimo, Márcio Gilberto-
Autor(es): dc.creatorFranco, Mauricio Machaim-
Autor(es): dc.creatorDode, Margot Alves Nunes-
Data de aceite: dc.date.accessioned2026-02-09T11:13:42Z-
Data de disponibilização: dc.date.available2026-02-09T11:13:42Z-
Data de envio: dc.date.issued2020-09-08-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2020-09-08-
Data de envio: dc.date.issued2023-06-27-
Data de envio: dc.date.issued2020-03-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/57396-
Fonte completa do material: dc.identifierhttps://doi.org/10.1071/RD19218-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1136376-
Descrição: dc.descriptionWe aimed to analyse the histone acetylation status and expression profile of genes involved in histone acetylation (histone acetyltransferase 1 (HAT1), lysine acetyltransferase 2A (KAT2A), histone deacetylase 1 (HDAC1), HDAC2 and HDAC3) in bovine oocytes of different competences during in vitro maturation (IVM). Cumulus–oocyte complexes were recovered from two groups of follicles: minor follicles (1.0–3.0 mm in diameter), classified as low competence (LC) and large follicles (6.0–8.0 mm in diameter) classified as high competence (HC). Oocytes were submitted to IVM for 0, 8 and 24 h and stored for analysis. Acetylation status of histone H4 on lysine K5, K6, K12 and K16 was assessed by immunohistochemistry. For gene expression, mRNA levels were determined by real-time quantitative polymerase chain reaction. All oocytes, regardless of their competence, showed a gradual decrease (P < 0.05) in acetylation signals during IVM. From 0 to 8 h of maturation, an increase (P < 0.05) in the relative abundance of HAT1 mRNA was observed only in the HC oocytes. In this group, higher (P < 0.05) mRNA levels of HDAC1 at 8 h of maturation were also observed. In conclusion, in the present study, LC oocytes were shown to have adequate acetylation levels for the resumption and progression of meiosis; however, these oocytes do not have the capacity to synthesise RNA during IVM as the HC oocytes do.-
Idioma: dc.languageen-
Publicador: dc.publisherCommonwealth Scientific and Industrial Research Organisation (CSIRO)-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceReproduction, Fertility and Development-
Palavras-chave: dc.subjectCumulus–oocyte complexes-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectHistone acetyltransferase-
Palavras-chave: dc.subjectImmunohistochemistry-
Palavras-chave: dc.subjectEpigenetic marks-
Palavras-chave: dc.subjectOócitos bovinos-
Palavras-chave: dc.subjectComplexos cúmulos-oócitos-
Palavras-chave: dc.subjectHistona acetiltransferase-
Palavras-chave: dc.subjectExpressão gênica-
Palavras-chave: dc.subjectImuno-histoquímica-
Palavras-chave: dc.subjectMarcas epigenéticas-
Título: dc.titleHistone acetylation during the in vitro maturation of bovine oocytes with different levels of competence-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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