Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorViveiros, Ana Tereza de Mendonça-
Autor(es): dc.creatorAmaral, Thiciana B-
Autor(es): dc.creatorOrfão, Laura Helena-
Autor(es): dc.creatorIsaú, Ziara Aparecida-
Autor(es): dc.creatorCaneppele, Danilo-
Autor(es): dc.creatorLeal, Marcelo de Castro-
Data de aceite: dc.date.accessioned2026-02-09T11:08:39Z-
Data de disponibilização: dc.date.available2026-02-09T11:08:39Z-
Data de envio: dc.date.issued2016-12-16-
Data de envio: dc.date.issued2016-12-16-
Data de envio: dc.date.issued2011-05-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/12080-
Fonte completa do material: dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2010.02761.x/full-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1134717-
Descrição: dc.descriptionThe aim of this study was to test the effects of cryoprotectants [dimethyl sulphoxide (DMSO) and methylglycol], extenders (0.9% NaCl, 5% glucose, Beltsville Thawing Solution™ and Merck III™), thawing temperatures (30 and 60 °C) and activating agents (0.29% NaCl and 1% NaHCO3) on the cryopreservation process of tiete tetra Brycon insignis sperm. Sperm was loaded in 0.5 mL straws, frozen in nitrogen vapour at −170 °C and stored in liquid nitrogen. Post-thaw sperm quality was evaluated in terms of subjective motility rate, quality motility score (0=no movement; 5=rapidly swimming spermatozoa), duration of motility and vitality (eosin–nigrosin staining). Post-thaw sperm motility rate was greater in methylglycol (76–88%), compared with DMSO (23–59%). In general, the highest quality motility scores were observed when sperm was thawed at 30 °C and triggered in 1% NaHCO3 (3.5–4.3). Duration of motility was longer when triggered in 1% NaHCO3 (95–120 s) compared with 0.29% NaCl (69–107 s). Sperm vitality was not affected by any of the parameters tested and varied from 51% to 69% intact sperm. Brycon insignis sperm frozen in methylglycol combined with any of the extenders tested and using the methods described above yields motility above 57% and that should last long enough to fertilize oocytes.-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceAquaculture Research-
Título: dc.titleSperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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