Application of the response surface methodology for optimization of whey protein partitioning in PEG/phosphate aqueous two-phase system

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorAlcântara, Lizzy Ayra Pereira-
Autor(es): dc.creatorMinim, Luis Antonio-
Autor(es): dc.creatorMinim, Valéria Paula Rodrigues-
Autor(es): dc.creatorBonomo, Renata Cristina Ferreira-
Autor(es): dc.creatorSilva, Luis Henrique Mendes da-
Autor(es): dc.creatorSilva, Maria do Carmo Hespanhol da-
Data de aceite: dc.date.accessioned2026-02-09T11:08:24Z-
Data de disponibilização: dc.date.available2026-02-09T11:08:24Z-
Data de envio: dc.date.issued2020-01-30-
Data de envio: dc.date.issued2020-01-30-
Data de envio: dc.date.issued2011-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/38791-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1570023211003126?via%3Dihub-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1134638-
Descrição: dc.descriptionIn order to develop a new strategy for β-lactoglobulin (β-lg) removal from whey protein, partitioning of α-lactalbumin (α-la), β-lg and glycomacropeptide (Gmp) was studied using aqueous two phase systems (ATPS). A system composed of 13% (w/w) polyethylene glycol (PEG, average molar mass 2000 g/mol) and 13% (w/w) potassium phosphate was used at 25 °C. A central composite rotatable design (CCRD) associated to the response surface methodology (RSM) was applied to investigate the effects of NaCl concentration and pH on the partition of these proteins. It was found that α-la and Gmp partitioned to the top phase rich in PEG, whereas β-lg partitioned to the bottom phase rich in salt. According to the RSM, optimal conditions for β-lg removal where found where pH was equal to 6.7 and salt concentration was 0.35 mol/L. Under these conditions, the partition coefficient Kα was 0.48 and KGmp was 0.92. On the other hand, the partition coefficient Kβ was only 0.01. In such conditions β-lg preferentially concentrates in the bottom phase, while the top phase exclusively contains the proteins α-la and Gmp. Fractionation of the proteins from fresh whey was performed in a three stage cross-flow extraction system. The extraction yield for β-lg in the bottom phase was 97.3%, while the yields for α-la and Gmp in the top phase were 81.1% and 97.8%, respectively.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceJournal of Chromatography B-
Palavras-chave: dc.subjectAqueous two phase systems-
Palavras-chave: dc.subjectα-Lactalbumin-
Palavras-chave: dc.subjectβ-Lactoglobulin-
Palavras-chave: dc.subjectGlycomacropeptide-
Título: dc.titleApplication of the response surface methodology for optimization of whey protein partitioning in PEG/phosphate aqueous two-phase system-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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