Synthetic peptides selected by immunoinformatics as potential tools for the specific diagnosis of canine visceral leishmaniasis.

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Autor(es): dc.creatorMoreira, Gabriel Jose Lucas-
Autor(es): dc.creatorMaia, Rodrigo da Costa-
Autor(es): dc.creatorSoares, Nathália Caroline-
Autor(es): dc.creatorOstolin, Thais Lopes Valentim Di Paschoale-
Autor(es): dc.creatorVital, Wendel Coura-
Autor(es): dc.creatorSoares, Rodrigo Aguiar-
Autor(es): dc.creatorRuiz, Jeronimo Conceição-
Autor(es): dc.creatorResende, Daniela de Melo-
Autor(es): dc.creatorBrito, Rory Cristiane Fortes de-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorRoatt, Bruno Mendes-
Data de aceite: dc.date.accessioned2025-08-21T15:57:44Z-
Data de disponibilização: dc.date.available2025-08-21T15:57:44Z-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19731-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/microorganisms12050906-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1028656-
Descrição: dc.descriptionDiagnosing canine visceral leishmaniasis (CVL) in Brazil faces challenges due to the limitations regarding the sensitivity and specificity of the current diagnostic protocol. Therefore, it is urgent to map new antigens or enhance the existing ones for future diagnostic techniques. Immunoinformatic tools are promising in the identification of new potential epitopes or antigen candidates. In this study, we evaluated peptides selected by epitope prediction for CVL serodiagnosis in ELISA assays. Ten B-cell epitopes were immunogenic in silico, but two peptides (peptides No. 45 and No. 48) showed the best performance in vitro. The selected peptides, both individually and in combination, were highly diagnostically accurate, with sensitivities ranging from 86.4% to 100% and with a specificity of approximately 90%. We observed that the combination of peptides showed better performance when compared to peptide alone, by detecting all asymptomatic dogs, showing lower cross-reactivity in sera from dogs with other canine infections, and did not detect vaccinated animals. Moreover, our data indicate the potential use of immunoinformatic tools associated with ELISA assays for the selection and evaluation of potential new targets, such as peptides, applied to the diagnosis of CVL.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.-
Palavras-chave: dc.subjectCanine visceral leishmaniasis-
Palavras-chave: dc.subjectSerological diagnosis-
Palavras-chave: dc.subjectPeptides-based ELISA-
Palavras-chave: dc.subjectImmunoinformatic-
Título: dc.titleSynthetic peptides selected by immunoinformatics as potential tools for the specific diagnosis of canine visceral leishmaniasis.-
Aparece nas coleções:Repositório Institucional - UFOP

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