Regulation of the N-acetyl-beta-D-glucosaminidase produced by Trichoderma harzianum : evidence that cAMP controls the expression.

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Autor(es): dc.creatorSilva, Roberto do Nascimento-
Autor(es): dc.creatorSilva, Silvana Petrofeza da-
Autor(es): dc.creatorBrandão, Rogélio Lopes-
Autor(es): dc.creatorUlhoa, Cirano José-
Data de aceite: dc.date.accessioned2025-08-21T15:56:34Z-
Data de disponibilização: dc.date.available2025-08-21T15:56:34Z-
Data de envio: dc.date.issued2017-03-20-
Data de envio: dc.date.issued2017-03-20-
Data de envio: dc.date.issued2004-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/7410-
Fonte completa do material: dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0923250804001494?np=y-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.resmic.2004.05.012-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1028103-
Descrição: dc.descriptionTrichoderma harzianum is a filamentous fungus reported to be a producer of extracellular N-acetyl-β-D-glucosaminidase (NAGase) when grown in chitin-containing medium. An approximately 64-kDa protein with NAGase activity was purified by gel filtration and ion exchange chromatography. The involvement of cyclic AMP (cAMP) in the synthesis of NAGase from T. harzianum in chitin-containing medium was also investigated. Molecules that increase the intracellular levels of cAMP, including caffeine, aluminium tetrafluoride and dinitrophenol, were used. Western blot analysis showed that NAGase synthesis was repressed by increasing the levels of intracellular cAMP. Using specific nag primers in a reverse transcription-polymerase chain reaction-based approach, NAGase synthesis was shown to be regulated at the level of gene transcription.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Título: dc.titleRegulation of the N-acetyl-beta-D-glucosaminidase produced by Trichoderma harzianum : evidence that cAMP controls the expression.-
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