Diagnosis of canine visceral leishmaniasis by flow cytometry serology using the rMELEISH multiepitope antigen coupled in a functional bead.

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Autor(es): dc.creatorMoura, Henrique Bonifácio-
Autor(es): dc.creatorKer, Henrique Gama-
Autor(es): dc.creatorRamos, Luana de Sousa-
Autor(es): dc.creatorNogueira, Lais Moreira-
Autor(es): dc.creatorMachado, Juliana Martins-
Autor(es): dc.creatorDias, Daniel Silva-
Autor(es): dc.creatorRodrigues, Rita Alaíde Leandro-
Autor(es): dc.creatorPaz, Mariana Campos da-
Autor(es): dc.creatorTeixeira Neto, Rafael Gonçalves-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorGaldino, Alexsandro Sobreira-
Autor(es): dc.creatorSilva, Eduardo Sérgio da-
Data de aceite: dc.date.accessioned2025-08-21T15:54:24Z-
Data de disponibilização: dc.date.available2025-08-21T15:54:24Z-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19730-
Fonte completa do material: dc.identifierhttps://www.eurekaselect.com/article/138739-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2174/0113892010268142231226115140-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1027114-
Descrição: dc.descriptionBackground: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite. Objective: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis. Methods: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH. Results: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs’ clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity. Conclusion: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectLeishmania infantum-
Palavras-chave: dc.subjectCanine visceral leishmaniasis-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectDogs-
Palavras-chave: dc.subjectFlow cytometry serology-
Título: dc.titleDiagnosis of canine visceral leishmaniasis by flow cytometry serology using the rMELEISH multiepitope antigen coupled in a functional bead.-
Aparece nas coleções:Repositório Institucional - UFOP

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