Ectonucleotidases from trypomastigotes from different sources and various genetic backgrounds of Trypanosoma cruzi potentiate their infectivity and host inflammation.

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Autor(es): dc.creatorLeite, Ana Luísa Junqueira-
Autor(es): dc.creatorOliveira, Daniela Silva de-
Autor(es): dc.creatorMota, Ludmilla Walter Reis-
Autor(es): dc.creatorCarvalho, Luana Cristina Faria-
Autor(es): dc.creatorZimmermann, Fernanda Francine-
Autor(es): dc.creatorPaiva, Nívia Carolina Nogueira de-
Autor(es): dc.creatorVieira, Paula Melo de Abreu-
Autor(es): dc.creatorLana, Marta de-
Autor(es): dc.creatorAfonso, Luís Carlos Crocco-
Autor(es): dc.creatorSilva, André Talvani Pedrosa da-
Data de aceite: dc.date.accessioned2025-08-21T15:51:50Z-
Data de disponibilização: dc.date.available2025-08-21T15:51:50Z-
Data de envio: dc.date.issued2021-09-23-
Data de envio: dc.date.issued2021-09-23-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/13787-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S1043466620302714?via%3Dihub-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.cyto.2020.155255-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1026069-
Descrição: dc.descriptionDistinct populations of Trypanosoma cruzi interact with mammalian cardiac muscle cells causing different inflammation patterns and low heart functionality. During T. cruzi infection, the extracellular ATP is hydrolyzed to tri- and/or diphosphate nucleotides, based on the infectivity, virulence, and regulation of the inflammatory response. T. cruzi carries out this hydrolysis through the T. cruzi ectonucleotidase, NTPDase-1 (TcNTPDase-1). This study aimed to evaluate the role of TcNTPDase-1 in culture rich in metacyclic trypomastigote forms (MT) and cell culture-derived trypomastigote forms (CT) from Colombiana (discrete typing unit - DTU I), VL-10 (DTU II), and CL (DTU VI) strains of T. cruzi. For this, we measured TcNTPDase-1 activity in suramin-treated and untreated parasites and infected J774 cells and C57BL/6 mice with suramin pre-treated parasites to assess parasitic and inflammatory cardiac profile in the acute phase of infection. Our data indicated a higher TcNTPDase-1 activity for ATP in culture rich in metacyclic trypomastigote forms from Colombiana strain in comparison to those from VL-10 and CL strains. The cell culture-derived trypomastigote forms from CL strain presented higher capacity to hydrolyze ATP than those from Colombiana and VL-10 strains. Suramin inhibited ATP hydrolysis in all studied parasite forms and strains. Suramin pre-treated parasites reduced J774 cell infection and increased nitrite production in vitro. In vivo studies showed a reduction of inflammatory infiltrate in the cardiac tissues of animals infected with cell culture-derived trypomastigote forms from suramin pre-treated Colombiana strain. In conclusion, TcNTPDase-1 activity in trypomastigotes forms drives part of the biological characteristics observed in distinct DTUs and may induce cardiac pathogenesis during T. cruzi infection.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectSuramin-
Título: dc.titleEctonucleotidases from trypomastigotes from different sources and various genetic backgrounds of Trypanosoma cruzi potentiate their infectivity and host inflammation.-
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