Detection of the antiviral activity of epicatechin isolated from Salacia crassifolia (Celastraceae) against Mayaro virus based on protein C homology modelling and virtual screening.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorFerreira, Priscila Gonçalves-
Autor(es): dc.creatorFerraz, Ariane Coelho-
Autor(es): dc.creatorFigueiredo, Jenyfer Emanuelle-
Autor(es): dc.creatorLima, Caroline Ferrante-
Autor(es): dc.creatorRodrigues, Vanessa Gregório-
Autor(es): dc.creatorTaranto, Alex Gutterres-
Autor(es): dc.creatorFerreira, Jaqueline Maria Siqueira-
Autor(es): dc.creatorBrandão, Geraldo Célio-
Autor(es): dc.creatorVieira Filho, Sidney Augusto-
Autor(es): dc.creatorDuarte, Lucienir Pains-
Autor(es): dc.creatorMagalhães, Cíntia Lopes de Brito-
Autor(es): dc.creatorMagalhães, José Carlos de-
Data de aceite: dc.date.accessioned2025-08-21T15:49:29Z-
Data de disponibilização: dc.date.available2025-08-21T15:49:29Z-
Data de envio: dc.date.issued2018-10-11-
Data de envio: dc.date.issued2018-10-11-
Data de envio: dc.date.issued2018-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/10347-
Fonte completa do material: dc.identifierhttps://link.springer.com/article/10.1007%2Fs00705-018-3774-1-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1025024-
Descrição: dc.descriptionMayaro fever, caused by Mayaro virus (MAYV) is a sub-lethal disease with symptoms that are easily confused with those of dengue fever, except for polyarthralgia, which may culminate in physical incapacitation. Recently, outbreaks of MAYV have been documented in metropolitan areas, and to date, there is no therapy or vaccine available. Moreover, there is no information regarding the three-dimensional structure of the viral proteins of MAYV, which is important in the search for antivirals. In this work, we constructed a three-dimensional model of protein C of MAYV by homology modelling, and this was employed in a manner similar to that of receptors in virtual screening studies to evaluate 590 molecules as prospective antiviral agents. In vitro bioassays were utilized to confirm the potential antiviral activity of the flavonoid epicatechin isolated from Salacia crassifolia (Celastraceae). The virtual screening showed that six flavonoids were promising ligands for protein C. The bioassays showed potent antiviral action of epicatechin, which protected the cells from almost all of the effects of viral infection. An effective concentration (EC50) of 0.247 μmol/mL was observed with a selectivity index (SI) of 7. The cytotoxicity assay showed that epicatechin has low toxicity, with a 50% cytotoxic concentration (CC50) greater than 1.723 µmol/mL. Epicatechin was found to be twice as potent as the reference antiviral ribavirin. Furthermore, a replication kinetics assay showed a strong inhibitory effect of epicatechin on MAYV growth, with a reduction of at least four logs in virus production. Our results indicate that epicatechin is a promising candidate for further testing as an antiviral agent against Mayaro virus and other alphaviruses.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Título: dc.titleDetection of the antiviral activity of epicatechin isolated from Salacia crassifolia (Celastraceae) against Mayaro virus based on protein C homology modelling and virtual screening.-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.