Refinement of the rKLi8.3-based serodiagnostic ELISA allows detection of canine leishmaniosis in dogs with low antibody titers.

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Autor(es): dc.creatorTeixeira, Henrique Couto-
Autor(es): dc.creatorValle, Giulia Pereira Costa-
Autor(es): dc.creatorMahdavi, Rouzbeh-
Autor(es): dc.creatorDias, Priscila Santos Martins-
Autor(es): dc.creatorOliveira, Erick Esteves de-
Autor(es): dc.creatorAira, Cristina P.-
Autor(es): dc.creatorHeinz, Daniela-
Autor(es): dc.creatorLatz, Andreas-
Autor(es): dc.creatorLana, Marta de-
Autor(es): dc.creatorMorgado, Fernanda Nazaré-
Autor(es): dc.creatorAlmeida, Renato Porrozzi de-
Autor(es): dc.creatorSteinhoff, Ulrich-
Data de aceite: dc.date.accessioned2025-08-21T15:48:38Z-
Data de disponibilização: dc.date.available2025-08-21T15:48:38Z-
Data de envio: dc.date.issued2025-03-10-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19885-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/pathogens13030246-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1024659-
Descrição: dc.descriptionThe diagnosis of canine leishmaniasis (CanL) still represents a challenge due to the variable clinical manifestations and the large number of asymptomatic dogs. Serological tests are most commonly used to detect infected animals, revealing anti-Leishmania antibodies, mainly of the IgG isotype. Recently, a new diagnostic antigen, rKLi8.3, containing 8.3 kinesin tandem repeats (TR) from a Leishmania infantum strain from Sudan, has been shown to provide excellent specificity and sensitivity for the detection of Leishmania-infected humans and dogs. However, asymptomatic animals with very low antibody titers are often difficult to detect by serodiagnosis. Thus, we wondered whether the addition of an anti-IgG-enhancing step in the protein A/G-based rKLi8.3-ELISA will improve the diagnostic performance without decreasing the specificity. For this, parasitologically confirmed CanL cases with low or high clinical scores, uninfected healthy controls and dogs with other infections were tested by rKLi8.3-ELISA as well as two different immunochromatographic rapid tests, rKLi8.3-lateral flow test (LFT) and Dual Path Platform (DPP®) based on the rK28 antigen. Our results show that the diagnostic accuracies of the rKLi8.3-ELISA and LFT were similar to that of DPP, missing several asymptomatic animals. However, the addition of a secondary, amplifying anti-dog IgG antibody in the protein A/G-based rKLi8.3-ELISA enabled the detection of nearly all asymptomatic dogs without compromising its specificity.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.-
Palavras-chave: dc.subjectLeishmaniasis-
Palavras-chave: dc.subjectCanine leishmaniasis-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectRecombinant proteins-
Palavras-chave: dc.subjectKinesins-
Título: dc.titleRefinement of the rKLi8.3-based serodiagnostic ELISA allows detection of canine leishmaniosis in dogs with low antibody titers.-
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