Mechanistic insights into the intracellular release of doxorubicin from pH-sensitive liposomes.

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Autor(es): dc.creatorReis, Samara Bonesso dos-
Autor(es): dc.creatorSilva, Juliana de Oliveira-
Autor(es): dc.creatorFossa, Fernanda Garcia-
Autor(es): dc.creatorLeite, Elaine Amaral-
Autor(es): dc.creatorSouza, Angelo Malachias de-
Autor(es): dc.creatorLana, Gwenaelle Elza Nathalie Pound-
Autor(es): dc.creatorMosqueira, Vanessa Carla Furtado-
Autor(es): dc.creatorOliveira, Mônica Cristina de-
Autor(es): dc.creatorBarros, André Luís Branco de-
Autor(es): dc.creatorJesus, Marcelo Bispo de-
Data de aceite: dc.date.accessioned2025-08-21T15:48:23Z-
Data de disponibilização: dc.date.available2025-08-21T15:48:23Z-
Data de envio: dc.date.issued2021-12-06-
Data de envio: dc.date.issued2021-12-06-
Data de envio: dc.date.issued2020-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/14120-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.biopha.2020.110952-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1024541-
Descrição: dc.descriptionpH-sensitive liposomes are interesting carriers for drug-delivery, undertaking rapid bilayer destabilization in response to pH changes, allied to tumor accumulation, a desirable behavior in the treatment of cancer cells. Previously, we have shown that pH-sensitive liposomes accumulate in tumor tissues of mice, in which an acidic environment accelerates drug delivery. Ultimately, these formulations can be internalized by tumor cells and take the endosome-lysosomal route. However, the mechanism of doxorubicin release and intracellular traffic of pH-sensitive liposomes remains unclear. To investigate the molecular mechanisms underlying the intracellular release of doxorubicin from pH-sensitive liposomes, we followed HeLa cells viability, internalization, intracel lular trafficking, and doxorubicin’s intracellular delivery mechanisms from pH-sensitive (SpHL-DOX) and non pH-sensitive (nSpHL-DOX) formulations. We found that SpHL-DOX has faster internalization kinetics and intracellular release of doxorubicin, followed by strong nuclear accumulation compared to nSpHL-DOX. The increased nuclear accumulation led to the activation of cleaved caspase-3, which efficiently induced apoptosis. Remarkably, we found that chloroquine and E64d enhanced the cytotoxicity of SpHL-DOX. This knowledge is paramount to improve the efficiency of pH-sensitive liposomes or to be used as a rational strategy for developing new formulations to be applied in vivo.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Fonte: o PDF do artigo.-
Palavras-chave: dc.subjectDrug delivery system-
Título: dc.titleMechanistic insights into the intracellular release of doxorubicin from pH-sensitive liposomes.-
Aparece nas coleções:Repositório Institucional - UFOP

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