A Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorRibeiro, Patrícia Aparecida Fernandes-
Autor(es): dc.creatorDias, Daniel Silva-
Autor(es): dc.creatorNovais, Marcus V. M.-
Autor(es): dc.creatorLage, Daniela Pagliara-
Autor(es): dc.creatorTavares, Grasiele de Sousa Vieira-
Autor(es): dc.creatorMendonça, Débora Vasconcelos Costa-
Autor(es): dc.creatorOliveira, Jamil Silvano de-
Autor(es): dc.creatorChávez Fumagalli, Miguel Angel-
Autor(es): dc.creatorRoatt, Bruno Mendes-
Autor(es): dc.creatorDuarte, Mariana Costa-
Autor(es): dc.creatorSouza, Daniel Menezes-
Autor(es): dc.creatorRibeiro, Fernanda Ludolf-
Autor(es): dc.creatorTavares, Carlos Alberto Pereira-
Autor(es): dc.creatorOliveira, Mônica Cristina de-
Autor(es): dc.creatorCoelho, Eduardo Antônio Ferraz-
Data de aceite: dc.date.accessioned2025-08-21T15:44:32Z-
Data de disponibilização: dc.date.available2025-08-21T15:44:32Z-
Data de envio: dc.date.issued2019-04-02-
Data de envio: dc.date.issued2019-04-02-
Data de envio: dc.date.issued2018-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/10910-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1043466618303557-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1023009-
Descrição: dc.descriptionLeishmania proteins have been evaluated as vaccine candidates against leishmaniasis; however, most antigens present low immunogenicity and need to be added with immune adjuvants. A low number of licensed adjuvants exist on the market today; therefore, research conducted to produce new products is desirable. The present study sought to evaluate the immunogenicity and protective efficacy of a recombinant Leishmania hypothetical protein, namely LiHyR, administered with saponin or liposomes in BALB/c mice. Immunological and parasitological parameters were evaluated, and results showed significant protection against Leishmania infantum infection produced by both compositions in the immunized animals; however, this was not identified when the antigen was used alone. In addition, the liposomal formulation was more effective in inducing a polarized Th1 response in the vaccinated animals, which was maintained after challenge and reflected by lower parasitism found in all evaluated organs when the limiting dilution technique and RT-PCR assay were employed. The protected animals showed higher levels of protein and parasite-specific IFN-γ IL-2, IL-12, GM-CSF, and TNF-α, which were evaluated by capture ELISA and flow cytometry, in addition to a higher production of anti-protein and anti-parasite IgG2a antibodies, both before and after challenge. The Lip/rLiHyR combination induced higher IFN-γ production through both CD4+ and CD8+ T cell subtypes. Results indicate the possibility of using the LiHyR, containing a liposomal formulation, as a vaccine candidate against visceral leishmaniasis.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectAdjuvants-
Palavras-chave: dc.subjectVaccine-
Palavras-chave: dc.subjectSaponin-
Título: dc.titleA Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis.-
Aparece nas coleções:Repositório Institucional - UFOP

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