Pristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus.

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Autor(es): dc.creatorNizer, Waleska Stephanie da Cruz-
Autor(es): dc.creatorFerraz, Ariane Coelho-
Autor(es): dc.creatorMoraes, Thaís de Fátima Silva-
Autor(es): dc.creatorLima, William Gustavo de-
Autor(es): dc.creatorSantos, Josana Pereira dos-
Autor(es): dc.creatorDuarte, Lucienir Pains-
Autor(es): dc.creatorFerreira, Jaqueline Maria Siqueira-
Autor(es): dc.creatorMagalhães, Cíntia Lopes de Brito-
Autor(es): dc.creatorVieira Filho, Sidney Augusto-
Autor(es): dc.creatorAndrade, Ana Cláudia dos Santos Pereira-
Autor(es): dc.creatorRodrigues, Rodrigo Araújo Lima-
Autor(es): dc.creatorAbrahão, Jônatas Santos-
Autor(es): dc.creatorMagalhães, José Carlos de-
Data de aceite: dc.date.accessioned2025-08-21T15:44:01Z-
Data de disponibilização: dc.date.available2025-08-21T15:44:01Z-
Data de envio: dc.date.issued2021-10-17-
Data de envio: dc.date.issued2021-10-17-
Data de envio: dc.date.issued2020-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/13878-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S0378874120333080-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.jep.2020.113423-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1022799-
Descrição: dc.descriptionEthnopharmacological relevance: Pristimerin is a triterpenoid considered the main component of Salacia crassifolia extracts. This terpene has shown promising antitumor, anti-inflammatory, and antimicrobial effects. Likewise, S. crassifolia has been used in traditional medicine to treat cancer and as an antimicrobial and anti-inflammatory agent. Aim of the study: This study aimed to evaluate the antibacterial activity of the hexane extract of Salacia crassifolia roots (HER) and its isolate, pristimerin, against pathogenic bacteria. Materials and methods: First, we evaluated the spectrum of action of HER and pristimerin by the determination of the minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). Subsequently, we analyzed the time-kill curve of these plant-derived compounds against Staphylococcus aureus. Then, we examined their mode of action by three different assays: the crystal violet methodology, the release of intracellular material, and transmission electron microscopy methods (TEM). Finally, we evaluated the effect of HER and pristimerin on the pre-formed biofilm of S. aureus by the crystal violet assay, the synergistic effect by the checkerboard method, the cytotoxicity against Vero cells, and the in silico activity using the online software PASS. Results: HER and pristimerin presented a narrow spectrum of action against Gram-positive bacteria (MIC 0.195–25 μg/mL), and their primary mode of action is the alteration of membrane permeability of S. aureus. Our results show that the compounds disrupted the pre-formed biofilm of S. aureus in a dose-dependent manner. Furthermore, HER and pristimerin presented a significant synergic effect after the combination with well-known antibiotics, which was associated with the ability of these phytomedicines to change membrane permeability. Regarding the cytotoxic effect, the selective index (SI) of HER ranged from 0.37 to 11.86, and the SI of pristimerin varied from 0.24 to 30.87, according to the bacteria tested. Conclusions: Overall, HER and pristimerin showed a promising antibacterial effect in vitro through the alteration of membrane permeability of S. aureus.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectSynergic effect-
Título: dc.titlePristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus.-
Aparece nas coleções:Repositório Institucional - UFOP

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