Effects of continuous millifluidic process on the physicochemical properties and freeze-drying of cloxacillin benzathine-loaded nanocapsules.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorPereira, Douglas Gualberto Sales-
Autor(es): dc.creatorTorquato, Laura Cota-
Autor(es): dc.creatorMarques, Maria Betânia de Freitas-
Autor(es): dc.creatorSenna, Carlos Alberto-
Autor(es): dc.creatorMenezes, Rodrigo Prioli-
Autor(es): dc.creatorMosqueira, Vanessa Carla Furtado-
Autor(es): dc.creatorAraújo, Raquel Silva-
Data de aceite: dc.date.accessioned2025-08-21T15:43:41Z-
Data de disponibilização: dc.date.available2025-08-21T15:43:41Z-
Data de envio: dc.date.issued2025-03-07-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19865-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1773224724006385-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.jddst.2024.105969-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1022649-
Descrição: dc.descriptionPoly-ε-caprolactone nanocapsules loading cloxacillin benzathine (CLOXB-NC) were produced by batchnanoprecipitation (BNP) and millifluidic continuous flow (CNP) methods. Regarding the CNP, a new millifluidics device was constructed, and the effects of flow rate and organic to aqueous phase proportions (OP/AP) were investigated. Dynamic light scattering (DLS) and atomic force microscopy (AFM) were used to characterize the NC size distribution and morphology, respectively. CLOXB was quantified by high-performance liquid chromatography (HPLC) to determine encapsulation efficiency (EE) and loading yield. DLS results show that unloaded NC can be produced with a narrow size distribution by CNP with an AP flow of 77 mL/min with an OP/ AP of 1:2 by CNP. Based on optimized conditions, CLOXB-NC was produced and compared with the BNP method. CLOXB-NC produced by CNP was spherical and presented a higher EE and a smaller distribution size than NC produced by BNP. Freeze-drying was carried out under two freezing conditions, - 20 ◦C and fast frozen in liquid nitrogen (- 196 ◦C). DLS, HPLC, AFM, SEM, and thermal analysis were applied to characterize the freeze-dried formulations. Both freezing conditions were satisfactory for producing CLOXB-NC powders using sucrose as a cryoprotectant. After powder resuspension, CLOXB-NCs frozen in liquid nitrogen showed superior drug leakage than samples frozen at - 20 ◦C. Interestingly, CLOXB-NCs produced by CNP tolerated the freeze-drying process better. NC loading CLOXB (0.5 mg/mL) produced by CNP and frozen at - 20 ◦C with 10 % (p/v) of sucrose demonstrated Sf/Si closest to 1, less drug leakage, fluffy and porous cake. In conclusion, this novel approach to producing NC in continuous flow in a simple and accessible millifluidic device represents an innovative process to improve NC’s aptness to withstand mechanical stress during the freeze-drying process.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectPolymeric nanocapsule-
Palavras-chave: dc.subjectBeta-lactam drug-
Palavras-chave: dc.subjectFreeze-drying-
Palavras-chave: dc.subjectSucrose-
Palavras-chave: dc.subjectMillifluidics-
Título: dc.titleEffects of continuous millifluidic process on the physicochemical properties and freeze-drying of cloxacillin benzathine-loaded nanocapsules.-
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