Leishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorPortela, Ricardo Wagner Dias-
Autor(es): dc.creatorSoares, Rodrigo Pedro Pinto-
Autor(es): dc.creatorPorfírio Passos, Gabriela-
Autor(es): dc.creatorLarangeira, Daniela Farias-
Autor(es): dc.creatorBarral, Thiago Assis Doria-
Autor(es): dc.creatorSampaio, Julia Ramos-
Autor(es): dc.creatorBernardo, Marcos-
Autor(es): dc.creatorSobrinho, Edneia Venâncio Alves-
Autor(es): dc.creatorBahia, Maria Terezinha-
Autor(es): dc.creatorPinho, Flaviane Alves-
Autor(es): dc.creatorMelo, Stella Maria Barrouin-
Data de aceite: dc.date.accessioned2025-08-21T15:43:11Z-
Data de disponibilização: dc.date.available2025-08-21T15:43:11Z-
Data de envio: dc.date.issued2020-04-17-
Data de envio: dc.date.issued2020-04-17-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/12077-
Fonte completa do material: dc.identifierhttps://doi.org/10.1371/journal.pntd.0007720-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1022452-
Descrição: dc.descriptionLipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania protozoan and has an important biological role in host-parasite interactions both in the midgut epithelium of the sand fly vector and in the vertebrate macrophages. Canine leishmaniasis (CanL) is a chronic infectious disease predominantly caused by Leishmania infantum. An early and accurate immunodiagnosis of the disease is crucial for veterinary clinical practice and for disease control. In this work, we evaluated L. infantum LPG as an antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for CanL immunodiagnosis (LPG-ELISA) by testing serum samples from 97 naturally infected dogs with diverse clinical presentations ranging from subclinical infection to severe disease, as evaluated by veterinarian infectologists. Serum samples from healthy dogs from non-endemic areas (n = 68) and from dogs with other infectious diseases (n = 64) were used as controls for assay validation. The performance of the LPG-ELISA was compared with that of an ELISA using the soluble fraction of L. infantum total lysate antigen (TLA). LPG-ELISA presented a superior performance in comparison to TLA-ELISA, with 91.5% sensitivity, 98.5% specificity and 99.7% accuracy. A distinguishing feature of the LPG-ELISA compared to the TLA-ELISA was its higher ability to identify subclinical infection in clinically healthy dogs, in addition to the absence of cross-reactivity with other canine infectious diseases. Finally, LPG-ELISA was compared to TR DPP visceral canine leishmaniasis test, the immunochromatographic test recommended by the Brazilian Ministry of Agriculture. LPG-ELISA exhibited higher values of specificity (98.5% versus 93.1%) and sensitivity (91.5% versus 90.6%) compared to TR DPP. In conclusion, L. infantum-derived LPG was recognized by antibodies elicited during CanL in different infection stages and was shown to be a suitable antigen for specific clinical settings of veterinary diagnosis and for public health usage.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.-
Título: dc.titleLeishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis.-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.