Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorAraújo, Glaucy Rodrigues de-
Autor(es): dc.creatorRabelo, Ana Carolina Silveira-
Autor(es): dc.creatorMeira, Janaína Serenato-
Autor(es): dc.creatorRossoni Júnior, Joamyr Victor-
Autor(es): dc.creatorBorges, William de Castro-
Autor(es): dc.creatorCota, Renata Guerra de Sá-
Autor(es): dc.creatorBatista, Maurício Azevedo-
Autor(es): dc.creatorLemos, Denise da Silveira-
Autor(es): dc.creatorSouza, Gustavo Henrique Bianco de-
Autor(es): dc.creatorBrandão, Geraldo Célio-
Autor(es): dc.creatorChaves, Míriam Martins-
Autor(es): dc.creatorCosta, Daniela Caldeira-
Data de aceite: dc.date.accessioned2025-08-21T15:42:54Z-
Data de disponibilização: dc.date.available2025-08-21T15:42:54Z-
Data de envio: dc.date.issued2017-11-27-
Data de envio: dc.date.issued2017-11-27-
Data de envio: dc.date.issued2017-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/9185-
Fonte completa do material: dc.identifierhttp://journals.sagepub.com/doi/pdf/10.1177/1535370216672749-
Fonte completa do material: dc.identifierhttps://doi.org/10.1177%2F1535370216672749-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1022327-
Descrição: dc.descriptionBaccharis trimera, popularly known as ‘‘carqueja’’, is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells. SK-Hep 1 cells were treated with B. trimera, quercetin, or rutin and then stimulated or notwith PMA/ionomycin and labeled with carboxy H2DCFDA for detection of reactive oxygen species by flow cytometer. The PKC expression by Western blot and enzyme activity was performed to evaluate the influence of B. trimera and quercetin on PKC signaling pathway. p47phox and p47phox phosphorylated expression was performed byWestern blot to evaluate the influence of B. trimera on p47phox phosphorylation. The results showed that cells stimulated with PMA/ionomycin (activators of PKC) showed significantly increased reactive oxygen species production, and this production returned to baseline levels after treatment with DPI (NOX inhibitor). Both B. trimera and quercetin modulated reactive oxygen species production through the inhibition of PKC protein expression and enzymatic activity, also with inhibition of p47phox phosphorylation. Taken together, these results suggest that B. trimera has a potentialmechanism for inhibiting reactive oxygen species production through the PKC signaling pathway and inhibition subunit p47phox phosphorylation of nicotinamide adenine dinucleotide phosphate oxidase.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectNicotinamide adenine dinucleotide phosphate oxidase-
Palavras-chave: dc.subjectReactive oxygen species-
Palavras-chave: dc.subjectQuercetin-
Palavras-chave: dc.subjectRutin-
Título: dc.titleBaccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells.-
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