Inhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorMurata, Ramiro Mendonça-
Autor(es): dc.creatorAlmeida, Luciana Salles Branco de-
Autor(es): dc.creatorYatsuda, Regiane-
Autor(es): dc.creatorSantos, Marcelo Henrique dos-
Autor(es): dc.creatorNagem, Tanus Jorge-
Autor(es): dc.creatorRosalen, Pedro Luiz-
Autor(es): dc.creatorKoo, Hyun-
Data de aceite: dc.date.accessioned2025-08-21T15:41:28Z-
Data de disponibilização: dc.date.available2025-08-21T15:41:28Z-
Data de envio: dc.date.issued2017-06-08-
Data de envio: dc.date.issued2017-06-08-
Data de envio: dc.date.issued2008-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/7923-
Fonte completa do material: dc.identifierhttps://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2008.01117.x-
Fonte completa do material: dc.identifierhttps://doi.org/10.1111/j.1574-6968.2008.01117.x-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1021676-
Descrição: dc.descriptionThe aim of this study was to examine the effects of 7-epiclusianone, a new prenylated benzophenone isolated from the plant Rheedia gardneriana, on some of the virulence properties of Streptococcus mutans associated with biofilm development and acidogenicity. The synthesis of glucans by glucosyltransferases B (GTF B) and C (GTF C) was markedly reduced by 7-epiclusianone showing more than 80% inhibition of enzymatic activity at a concentration of 100 mgmL 1. Doublereciprocal analysis (Lineweaver–Burk plots) revealed that the inhibition of GTF B activity was noncompetitive (mixed) while GTF C was inhibited uncompetitively. The glycolytic pH drop by S. mutans cells was also disrupted by 7-epiclusianone without affecting the bacterial viability, an effect that can be attributed, in part, to inhibition of F-ATPase activity (61.1 3.0% inhibition at 100 mgmL 1). Furthermore, topical applications (1-min exposure, twice daily) of 7-epiclusianone (at 250 mgmL 1) disrupted biofilm formation and physiology. The biomass (dryweight), extracellular insoluble polysaccharide concentration and acidogenicity of the biofilms were significantly reduced by the test agent (Po0.05). The data show that 7-epiclusianone disrupts the extracellular and intracellular sugar metabolism of S. mutans, and holds promise as a novel, naturally occurring compound to prevent biofilm-related oral diseases.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subject7-epiclusianone-
Palavras-chave: dc.subjectGlucosyltransferases-
Palavras-chave: dc.subjectGlycolysis-
Palavras-chave: dc.subjectStreptococcus mutans-
Título: dc.titleInhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans.-
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