Yellow fever virus infection in human hepatocyte cells triggers an imbalance in redox homeostasis with increased reactive oxygen species production, oxidative stress, and decreased antioxidant enzymes.

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Autor(es): dc.creatorFerraz, Ariane Coelho-
Autor(es): dc.creatorMenegatto, Marília Bueno da Silva-
Autor(es): dc.creatorLima, Rafaela Lameira Souza-
Autor(es): dc.creatorOla Olub, Oluwashola Samuel-
Autor(es): dc.creatorCosta, Daniela Caldeira-
Autor(es): dc.creatorMagalhães, José Carlos de-
Autor(es): dc.creatorRezende, Izabela Maurício-
Autor(es): dc.creatorLaBeaud, Angelle Desiree-
Autor(es): dc.creatorMonath, Thomas P.-
Autor(es): dc.creatorAlves, Pedro Augusto-
Autor(es): dc.creatorCarvalho, Andréa Teixeira de-
Autor(es): dc.creatorMartins Filho, Olindo Assis-
Autor(es): dc.creatorDrumond, Betânia Paiva-
Autor(es): dc.creatorMagalhães, Cíntia Lopes de Brito-
Data de aceite: dc.date.accessioned2025-08-21T15:31:48Z-
Data de disponibilização: dc.date.available2025-08-21T15:31:48Z-
Data de envio: dc.date.issued2025-02-27-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19809-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0891584924000509?via%3Dihub-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.freeradbiomed.2024.01.042-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1017540-
Descrição: dc.descriptionYellow fever (YF) presents a wide spectrum of severity, with clinical manifestations in humans ranging from febrile and self-limited to fatal cases. Although YF is an old disease for which an effective and safe vaccine exists, little is known about the viral- and host-specific mechanisms that contribute to liver pathology. Several studies have demonstrated that oxidative stress triggered by viral infections contributes to pathogenesis. We evaluated whether yellow fever virus (YFV), when infecting human hepatocytes cells, could trigger an imbalance in redox homeostasis, culminating in oxidative stress. YFV infection resulted in a significant increase in reactive oxygen species (ROS) levels from 2 to 4 days post infection (dpi). When measuring oxidative parameters at 4 dpi, YFV infection caused oxidative damage to lipids, proteins, and DNA, evidenced by an increase in lipid peroxidation/8- isoprostane, carbonyl protein, and 8-hydroxy-2′-deoxyguanosine, respectively. Furthermore, there was a significant reduction in the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), in addition to a reduction in the ratio of reduced to oxidized glutathione (GSH/GSSG), indicating a pro-oxidant environment. However, no changes were observed in the enzymatic activity of the enzyme catalase (CAT) or in the gene expression of SOD isoforms (1/2/3), CAT, or GPx. Therefore, our results show that YFV infection generates an imbalance in redox homeostasis, with the overproduction of ROS and depletion of antioxidant enzymes, which induces oxidative damage to cellular constituents. Moreover, as it has been demonstrated that oxidative stress is a conspicuous event in YFV infection, therapeutic strategies based on antioxidant biopharmaceuticals may be new targets for the treatment of YF.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectYellow fever virus-
Palavras-chave: dc.subjectRedox homeostasis-
Palavras-chave: dc.subjectReactive oxygen species-
Palavras-chave: dc.subjectAntioxidant defenses-
Palavras-chave: dc.subjectPathogenesis-
Título: dc.titleYellow fever virus infection in human hepatocyte cells triggers an imbalance in redox homeostasis with increased reactive oxygen species production, oxidative stress, and decreased antioxidant enzymes.-
Aparece nas coleções:Repositório Institucional - UFOP

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