Combination of high solid load, on‐site enzyme cocktails and surfactant in the hydrolysis of hydrothermally pretreated sugarcane bagasse and ethanol production.

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Autor(es): dc.creatorRodrigues, Patrísia de Oliveira-
Autor(es): dc.creatorMoreira, Felipe Santos-
Autor(es): dc.creatorCardoso, Vicelma Luiz-
Autor(es): dc.creatorSantos, Líbia Diniz-
Autor(es): dc.creatorGurgel, Leandro Vinícius Alves-
Autor(es): dc.creatorPasquini, Daniel-
Autor(es): dc.creatorBaffi, Milla Alves-
Data de aceite: dc.date.accessioned2025-08-21T15:30:52Z-
Data de disponibilização: dc.date.available2025-08-21T15:30:52Z-
Data de envio: dc.date.issued2023-05-23-
Data de envio: dc.date.issued2023-05-23-
Data de envio: dc.date.issued2021-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/16627-
Fonte completa do material: dc.identifierhttps://link.springer.com/article/10.1007/s12649-022-01685-1-
Fonte completa do material: dc.identifierhttps://doi.org/10.1007/s12649-022-01685-1-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1017127-
Descrição: dc.descriptionIn this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in sac- charifcations of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at time intervals of 12 h, using two homemade enzyme extracts (ES1 from Aspergillus niger monoculture and ES2 from A. niger, Trametes versicolor and Pleurotus ostreatus consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifcations were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a signifcant reduction of 69.26% in hemicel- luloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% signifcantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifcations of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1S and 24% in ES2S. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1S and 88.03% for F2S. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efciency of bioconversion of lignocellulosic biomass to fermentable sugars.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectHomemade enzyme extracts-
Palavras-chave: dc.subjectTriton X-100-
Título: dc.titleCombination of high solid load, on‐site enzyme cocktails and surfactant in the hydrolysis of hydrothermally pretreated sugarcane bagasse and ethanol production.-
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